Collagenase Iv
Collagenase Iv - Related Videos
09:41
Intrastriatal Injection of Autologous Blood or Clostridial Collagenase as Murine Models of Intracerebral Hemorrhage
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Cited by 35 •
2014
Preclinical models of intracerebral hemorrhage are utilized to mimic certain aspects of clinical disease. Thus, mechanisms of injury and potential therapeutic strategies may be explored. In this protocol, two models of intracerebral hemorrhage are described, intrastriatal (basal ganglia) injections of autologous blood or collagenase.
07:09
Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure
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Cited by 93 •
2013
A modified two-step collagenase perfusion procedure for isolation of human hepatocytes is described. This method can also be applied to other mammalian livers. The isolated hepatocytes are available in high yield and viability, making them a suitable model for scientific research in areas such as liver regeneration, pharmacokinetics and toxicology.
07:21
Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells
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2023
Pancreatic islets, also called the Islets of Langerhans, are a cluster of endocrine cells which produces hormones for glucose regulation and other important biological functions. This protocol explains a method for isolating pancreatic islets by first perfusing collagenase via Ampulla of Vater to digest the exocrine pancreatic tissue and a simplified density gradient centrifugation to purify the islets from mice.
10:44
Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase
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Cited by 60 •
2012
Clinically relevant animal models of intracerebral hemorrhage (ICH) are needed to extend our knowledge of hemorrhagic stroke and to examine novel therapeutic strategies. In this study, we describe and evaluate two ICH models that implement unilateral injections of either autologous whole blood or bacterial collagenase into the basal ganglia (corpus striatum) of mice.
06:59
Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease
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Cited by 103 •
2012
A detailed description of mouse islet isolation is described using the technique of in situ pancreatic ductal cannulation and perfusion of a combination of purified collagenase and neutral protease.
FAQs
Collagenase IV cleaves peptide bonds within collagen fibers, weakening the structural network that holds connective tissue together. This enzymatic action loosens the tissue sufficiently for embedded cells to be released under controlled laboratory conditions. Because the target is collagen, the treatment supports dissociation of complex tissues while allowing the resulting cell population to be collected for downstream analysis.
Buffered solutions under physiological or near-physiological conditions provide a controlled environment for tissue dissociation and help support cell viability during processing. Maintaining these conditions is particularly relevant when the released cells will undergo culture, flow cytometry, or functional assays. The goal is to weaken the collagen-rich matrix without compromising the usefulness of the isolated cells.
The extent of collagen breakdown determines how effectively cells are released from connective tissue and therefore influences the composition of the resulting preparation. Controlled dissociation is important because the intended outcome is a usable population of viable cells rather than indiscriminate tissue disruption. This balance enables researchers to examine cellular composition in organs, tumors, and infected tissues.
Breaking down collagen-rich tissue enables access to immune cells located within organs, tumors, and infected tissues. Researchers can then examine inflammatory cell recruitment, host-pathogen interactions, tissue remodeling, and disease-associated changes in cellular composition. The method therefore connects extracellular tissue structure with measurable immune and infection-related cellular responses.
A basic workflow places the tissue in a buffered solution containing Collagenase IV under physiological or near-physiological conditions, allowing collagen breakdown and controlled dissociation. The released cells are then used for laboratory analysis rather than remaining embedded in the original tissue matrix. The preparation can support subsequent flow cytometry, culture, or functional assays.
Cells obtained through this dissociation approach can be directed into flow cytometry, cell culture, or functional assays. Flow cytometry supports analysis of cellular populations, whereas culture and functional assays allow researchers to examine cell behavior or activity after isolation. Selecting among these outcomes depends on the biological question and the type of tissue being studied.
Researchers may use Collagenase IV when infected tissue must be dissociated to examine its cellular composition. Releasing cells from the collagen-supported matrix makes it possible to investigate immune responses in the context of infection, including host-pathogen interactions and inflammatory cell recruitment. The same preparation can provide material for flow cytometry, culture, or functional testing.
Dissociating collagen-containing tissue allows researchers to separate cellular analysis from the original structural matrix and assess which cell populations are present. In immunology and infection studies, this supports investigation of tissue remodeling and disease-associated shifts in cellular composition. Comparing preparations from relevant tissues can therefore connect structural changes with altered immune or infection-related cellular patterns.