Locked Nucleic Acid Oligonucleotides
Locked Nucleic Acid Oligonucleotides - Related Videos
07:25
Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides
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Cited by 3 •
2010
Oligonucleotides can be used to site specifically substitute a single nucleotide of transfected target genes in both Anopheles gambiae and Anopheles stephensi cells.
02:43
Nucleic Acids
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2020
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning. DNA and RNA The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
07:58
A Polyaniline-based Sensor of Nucleic Acids
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Cited by 12 •
2016
Nucleic acids are common analytes for assessing biological systems; however, bias from enzymatic manipulation can cause concern. Here a method is described for label-free detection of nucleic acids using polyaniline. This sensitive, cost-effective sensor technology can distinguish single nucleotide differences between molecules.
09:06
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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Cited by 1 •
2025
The protocol describes a tandem immunofluorescence hybridization assay with formalin-fixed paraffin-embedded tissue to detect and quantify microRNA and multiplexed proteins simultaneously.
09:45
Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection
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Cited by 11 •
2012
A novel high-throughput method is described that enables the detection and relative quantitation of small RNA and mRNA expression from single bacterial cells using locked nucleic acid probes and flow cytometry-fluorescence in situ hybridization.
FAQs
The methylene bridge linking the 2′ oxygen and 4′ carbon restricts the modified ribose to a conformation that favors base pairing. This structural constraint helps the strand recognize complementary DNA or RNA with high affinity and sequence specificity. In infection and immunology studies, that combination supports selective interaction with host, microbial, or pathogen-derived nucleic acid sequences.
High affinity strengthens binding to a complementary nucleic acid, while sequence specificity helps distinguish the intended DNA or RNA sequence from other nucleic acids. Together, these properties make the molecules useful when an experiment must focus on a selected gene, microRNA, or pathogen-derived sequence rather than broadly interacting with unrelated nucleic acids.
Improved resistance to enzymatic degradation can help Locked Nucleic Acid Oligonucleotides remain available for their intended nucleic acid interactions. This property complements their strong and selective binding, making them suitable for experiments that regulate gene expression, inhibit selected microRNAs, or detect pathogen-derived nucleic acids. The resulting tools can support more focused analysis of molecular events.
Antisense regulation uses a complementary oligonucleotide to influence expression from a selected nucleic acid target, whereas microRNA inhibition focuses specifically on blocking a selected microRNA. Locked Nucleic Acid Oligonucleotides can support both approaches because their sequence-directed binding enables researchers to choose particular gene-related or microRNA targets when examining host responses or infection-associated molecular changes.
Researchers can select sequences complementary to pathogen-derived DNA or RNA and use the resulting binding interaction to detect or characterize those nucleic acids. High affinity and sequence specificity are central to this application because they connect the observed signal or molecular interaction to a selected pathogen sequence. This helps investigate microbial genes and infection-related nucleic acid signatures.
In this subject area, the molecules provide tools for examining both sides of an infection-related system. They can regulate host gene expression, inhibit selected microRNAs involved in molecular responses, and detect or characterize pathogen-derived nucleic acids. These uses help connect specific nucleic acid sequences with host responses, microbial genes, and possible nucleic acid-based therapeutic strategies.