Visceral Leishmaniasis
Visceral Leishmaniasis - Related Videos
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Leishmaniasis
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2026
Leishmaniasis is a protozoal disease caused by species of the genus Leishmania and transmitted through the bite of infected female sandflies. The parasite exists in two principal morphological forms during its life cycle. A sandfly acquires intracellular amastigotes from an infected reservoir host, such as a dog. Within the sandfly, these forms differentiate into motile, flagellated promastigotes. During a subsequent blood meal, promastigotes are injected into the human host, where they...
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Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs
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Cited by 40 •
2012
Optimization of the experimental hamster model for cutaneous leishmaniasis by intradermal injection of Leishmania promastigotes at the dorsal skin. This approach is useful during inoculation, follow-up, characterization of lesions, application of treatments and obtaining of clinical samples. Locomotion, search for food and water, play and social activities are preserved.
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Controlled Reversible Visceral Arterial Ischemia, Venous Congestion and Combined Malperfusion via Midline Laparotomy in Rats
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2024
This article introduces a standardized procedure for controlled, reversible malperfusion of visceral organs in rat models. The aim is to induce these malperfusion states with a high degree of methodological certainty and control while maintaining technical simplicity and error resilience.
06:06
In Vivo Calcium Imaging of Dorsal Root Ganglia Neurons' Response to Somatic and Visceral Stimuli
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Cited by 2 •
2024
The present protocol outlines in vivo calcium imaging for measuring the responses of ensembles of lumbar-6 DRG neurons to somatic and visceral stimuli. Thorough comparisons can be made among neurons responding to different stimuli. This protocol is valuable for investigating mechanisms of visceral pain and somatic stimulation, such as acupuncture.
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In vivo Imaging of Transgenic Leishmania Parasites in a Live Host
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Cited by 65 •
2010
An in vivo imaging system is used to generate quantitative measurements of murine infection with the Trypanosomatid protozoan Leishmania. This is a non-invasive and non-lethal method for detecting parasites expressing luciferase within many tissues throughout the course of chronic Leishmania spp. infection.
FAQs
After entering macrophages, Leishmania parasites transform into amastigotes and multiply within phagolysosomes. This intracellular location is central to persistence because the organisms remain inside immune cells while reproducing. Continued infection can support movement beyond the initial site, helping explain why disease affects the spleen, liver, and bone marrow.
Host immunity has a dual significance in visceral leishmaniasis: it can limit parasite control, yet immune activity may also contribute to tissue damage. This balance helps explain why studying infection requires attention to both pathogen persistence and the host response, rather than treating symptoms as consequences of parasite growth alone.
The spleen, liver, and bone marrow are important sites for interpreting disease progression because involvement across these organs reflects systemic infection. Reduced blood cell counts provide an additional outcome to monitor alongside prolonged fever, weight loss, and organ enlargement. Together, these findings connect immune and parasitic processes with measurable clinical effects.
Improved diagnostic methods are important because visceral leishmaniasis combines intracellular parasite persistence with systemic effects. Diagnostic research therefore addresses more than a single symptom: it must help identify infection in the context of affected organs and findings such as prolonged fever, organ enlargement, and reduced blood cell counts. Specific tests depend on the research approach.
Antileishmanial treatment research is relevant not only to reducing clinical manifestations but also to addressing parasites that persist within macrophages. Vaccine strategies approach the problem earlier by seeking protective immune responses. Together, these applications reflect two complementary goals: controlling established infection and improving future host protection. The overview identifies both as major research directions.
Within immunology and infection, visceral leishmaniasis serves as a model for examining host-pathogen interactions in a systemic setting. Researchers can relate parasite survival in macrophages to immune control, tissue damage, organ involvement, and blood-cell changes. This integrated perspective helps connect cellular events at the phagolysosome with outcomes observed across the organism.