Bio-energetic Assay
Bio-energetic Assay - Related Videos
08:48
Bio-energetics Investigation of Candida albicans Using Real-time Extracellular Flux Analysis
0 Views •
Cited by 1 •
2019
Here, we present a stepwise protocol to investigate the mitochondrial respiration and glycolytic function in Candida Albicans using an extra flux analyzer.
09:50
Preparation and Reactivity of Gasless Nanostructured Energetic Materials
0 Views •
Cited by 5 •
2015
This protocol describes the preparation of gasless nanostructured energetic materials (Ni+Al, Ta+C, Ti+C) using the short-term high-energy ball milling (HEBM) technique. It also describes a high-speed thermal imaging method to study the reactivity of mechanically fabricated nanocomposites. These protocols can be extended to other reactive nanostructured energetic materials.
02:35
Energetics of Solution Formation
0 Views •
2023
The formation of a solution is an example of a spontaneous process, which is a process that occurs under specified conditions without energy from some external source. When the strengths of the intermolecular forces of attraction between solute and solvent species in a solution are no different than those present in the separated components, the solution is formed with no accompanying energy change. Formation of the solution requires the solute–solute and solvent–solvent electrostatic forces to...
11:38
Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy
0 Views •
Cited by 36 •
2010
Langendorff-mode isolated heart perfusion, in conjunction with 31P NMR spectroscopy, combines the fields of biochemistry and physiology into one experiment. The protocol allows for the dynamic measurement of high energy phosphate content and turnover in the heart while concurrently monitoring physiologic function. When performed correctly, this is a valuable technique in the assessment of cardiac energetics.
16:38
Bridging the Bio-Electronic Interface with Biofabrication
0 Views •
Cited by 3 •
2012
This article describes a biofabrication approach: deposition of stimuli-responsive polysaccharides in the presence of biased electrodes to create biocompatible films which can be functionalized with cells or proteins. We demonstrate a bench-top strategy for the generation of the films as well as their basic uses for creating interactive biofunctionalized surfaces for lab-on-a-chip applications.
FAQs
These measurements capture complementary features of cellular energetics. ATP levels indicate the available energy currency, oxygen consumption reflects mitochondrial respiration, and extracellular acidification indicates metabolic activity associated with glycolysis. Examining them together can help distinguish changes in energy production pathways and reveal whether cells alter respiration, glycolysis, or both under defined experimental conditions.
Comparing mitochondrial respiration with glycolytic activity shows how cells distribute energy production between major metabolic pathways. A change in one measurement may indicate a shift in metabolic activity rather than a general loss of function. This comparison is useful for characterizing cellular responses to physiological stress, disease-related dysfunction, or experimental treatment.
Defined conditions are important because cellular energy production responds to external influences, including physiological stress and drug exposure. Such influences can alter ATP levels, oxygen consumption, extracellular acidification, or the balance between respiration and glycolysis. Controlling and comparing these conditions allows investigators to associate a measured metabolic shift with a specific treatment or stress context.
A typical workflow begins with selecting cultured cells or tissue samples and establishing defined experimental conditions. Investigators then measure one or more energy-related indicators, such as ATP, oxygen consumption, or extracellular acidification, and compare the results across conditions. The resulting profiles are interpreted alongside cellular viability and function to assess metabolic changes.
Energy measurements become more informative when considered with cell viability and function. A metabolic change may reflect altered cellular activity, physiological stress, or impaired health, so the energy profile should not be viewed in isolation. Linking these observations helps investigators determine whether a treatment or condition changes metabolism while preserving, disrupting, or otherwise affecting cellular function.
Medical researchers use these assays to investigate metabolic dysfunction, drug responses, disease mechanisms, and physiological stress. Applications include studies of cancer, neurodegeneration, and inflammation, as well as therapeutic development. By measuring how cellular energy metabolism changes under defined conditions, the assays provide a way to connect disease or treatment effects with cellular function.