Cartridge-based Pcr System
Cartridge-based Pcr System - Related Videos
03:31
A Microfluidic Cartridge-Based Detection System for Allergen-Specific Immunoglobulin E
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2025
This video demonstrates a protocol for detecting serum-specific immunoglobulin E using a microfluidic cartridge-based chemiluminescence system. Serum IgEs interact with distinct allergens immobilized in the reaction zone of the cartridge, which are subsequently detected with peroxidase-conjugated anti-IgE antibodies and a chemiluminescent substrate, allowing simultaneous detection of multiple allergen-specific IgEs.
06:01
Optimized PCR-based Detection of Mycoplasma
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Cited by 14 •
2011
The LookOut Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma, Acholeplasma, and Ureaplasma contamination in cell cultures and other cell culture derived biologicals.
08:04
Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA
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Cited by 133 •
2016
We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.
02:25
PCR-Based Detection of Foodborne Pathogens from Enriched Fly Samples
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2026
Source: Pava-Ripoll, et al. Detection of Foodborne Bacterial Pathogens from Individual Filth Flies. J. Vis. Exp. (2015)This video demonstrates a PCR-based method that amplifies target bacterial DNA from enriched fly samples using pathogen-specific tablets, enabling real-time fluorescence detection and melting curve analysis to confirm the presence of foodborne pathogens in individual insects.
09:02
Rapid PCR Thermocycling using Microscale Thermal Convection
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Cited by 21 •
2011
We describe a novel method to perform DNA replication via the polymerase chain reaction (PCR). Thermal convection is harnessed to continuously shuttle reagents between denaturing, annealing, and extension conditions by maintaining opposing surfaces of the reactor at constant temperature. This inherently simple design promises to make rapid PCR more accessible.
FAQs
Thermal cycling repeatedly changes the reaction temperature so target DNA or complementary DNA can be amplified through successive cycles. Sequence-specific primers determine which nucleic acid sequence is copied, giving the assay its analytical specificity. Together, cycling conditions and primer design allow the system to distinguish the intended target before fluorescence-based detection reports the result.
Integrated chambers or channels organize the main analytical stages within the cartridge, including sample preparation, reagent mixing, thermal cycling, and detection. This architecture moves the specimen through a defined sequence without requiring separate manual transfers between instruments. Coordinating these stages inside the cartridge supports consistent processing and helps reduce hands-on variation between tests.
A sealed, disposable cartridge limits the need to open or transfer material after the specimen is introduced. That design reduces hands-on handling and helps limit contamination risk during amplification and detection. It also supports a more standardized workflow, which is particularly useful when testing must be performed quickly or outside a centralized laboratory environment.
Once the specimen enters, the cartridge carries it through sample preparation and reagent mixing before thermal cycling amplifies the selected nucleic acid target. The system then uses fluorescence-based detection to identify the amplified material. Because these stages are integrated, the workflow can produce a molecular result without requiring the operator to manage each analytical step separately.
In medicine, these systems can support infectious disease diagnosis, genetic testing, and detection of clinically important mutations. The same general platform therefore serves both pathogen-focused testing and analyses of human genetic material. Its value depends on the specific assay incorporated into the cartridge, including the primers and detection design selected for the clinical target.
Rapid, standardized molecular results can provide clinically useful information sooner, supporting more timely treatment decisions. Because testing can be performed in decentralized settings, access does not have to depend entirely on a central laboratory. This combination of speed, integrated processing, and reduced handling makes the platform relevant when diagnostic information is needed near the point of care.