Cecum Puncture
Cecum Puncture - Related Videos
11:53
Cecal Ligation Puncture Procedure
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Cited by 200 •
2011
The mouse model of cecal ligation and puncture as a valuable tool for the study of human sepsis.
04:47
Cardiac Blood Collection via Subclavian Puncture in Mice
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2025
Subclavian cardiac puncture is a simple, reliable method for collecting terminal blood in mice. It is easy to operate and ensures high-purity samples.
05:27
Drawing Blood from Rats through the Saphenous Vein and by Cardiac Puncture
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Cited by 87 •
2007
Blood draws are necessary in a large number of studies, for example to study the pharmacokinetics profile of a compound. Here, we demonstrate how to draw blood from rats using two techniques: blood draw from the saphenous vein or by cardiac puncture.
06:40
Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice
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Cited by 56 •
2014
Experimental sepsis can be induced in mice using the cecal ligation and puncture (CLP) method. Current protocols to assess autophagy in vivo in the context of CLP-induced sepsis are presented here: A protocol for measuring autophagy using (GFP)-LC3 mice, and a protocol for measuring autophagosome formation by electron microscopy.
08:00
Reduction of Iatrogenic Atrial Septal Defects with an Anterior and Inferior Transseptal Puncture Site when Operating the Cryoballoon Ablation Catheter
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Cited by 18 •
2015
The goal of this study is to demonstrate the preferential location of transseptal puncture during a cryoballoon catheter ablation procedure for the treatment of atrial fibrillation.
FAQs
Ligation establishes the controlled injury component of the model before the cecum is punctured. It helps create a defined setting in which material from the cecum can enter the peritoneal cavity after penetration. That design allows investigators to compare host responses and treatment strategies under a disease condition generated through the same general experimental framework.
The key biological consequence is exposure of the peritoneal cavity to both fecal material and intestinal bacteria. This polymicrobial contamination produces peritonitis, followed by systemic inflammation and potentially sepsis. The sequence makes it possible to examine how a localized breach of the intestine is associated with broader infection-related responses, including progression toward organ dysfunction in severe disease.
Because the model can produce systemic inflammation and potentially sepsis, it provides a preclinical setting for studying organ dysfunction associated with that disease state. Investigators can examine host responses across the progression from peritoneal contamination to systemic illness, then use those observations to compare disease mechanisms or assess whether interventions alter the resulting condition.
At a high level, the experimental workflow consists of establishing the cecal condition, penetrating it with a needle or other instrument, and allowing access to its contents or creating a controlled injury. In the ligation-and-puncture model, puncture follows ligation, and the resulting peritoneal exposure becomes the basis for subsequent medical or experimental assessment.
Researchers use this preclinical approach to evaluate antibiotics and supportive therapies. Antibiotics can be studied in relation to polymicrobial infection, while supportive approaches can be assessed against systemic inflammation, sepsis, or associated organ dysfunction. The controlled design also supports comparisons between treatment strategies rather than limiting investigation to disease description alone.
The same general model can generate a controlled injury and a defined route for intestinal contents to enter the peritoneal cavity. Because this leads to relevant disease features such as peritonitis, systemic inflammation, potential sepsis, and organ dysfunction, researchers can compare how different mechanisms or interventions influence host responses and disease outcomes.