Corneoscleral Double Marking

Corneoscleral double marking is an ophthalmic technique that establishes a reliable ocular reference axis for procedures requiring precise meridional alignment, particularly toric intraocular lens implantation. The method places corresponding reference marks on the cornea and adjacent sclera, allowing the intended axis to be identified and transferred accurately despite changes in patient position or ocular rotation during surgery. By improving alignment between the toric lens and the patient’s astigmatic axis, this approach can enhance refractive precision and reduce postoperative residual astigmatism. It is therefore useful in cataract surgery and other procedures in which small rotational errors can compromise visual outcomes.

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JoVE EoE - Large Animal Models

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2025

In this video, we show a procedure to surgically isolate the corneoscleral button to generate an ex vivo porcine cornea model. The model is helpful for histological, pathological, and pharmacological studies due to its similarity to the human eye.

Research

JoVE Journal - Bioengineering
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The Bionic Clicker Mark I & II

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Cited by 2 •

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A device was created to demonstrate electromyography-based control to a lay audience. After the success of the initial device, a second device was made with greater flexibility in functionality for demonstration and research purposes. This protocol describes the process of building and calibrating both devices.

Education

JoVE Core - Anatomy and Physiology

Bone Markings

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2025

Bones have various surface features that help form joints and attach to other soft tissues. Depending on the function, bone markings are categorized into articulating projections, processes for attachment, depressions, and openings. Articulating Projections Articulating projections are found where two bones meet to form a joint. These structures are usually found at the ends of bones. The largest articulation is a rounded projection called the head, supported by a narrow neck at the ends of...

Administering and Detecting Protein Marks on Arthropods for Dispersal Research

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Cited by 12 •

2016

Proteins are often used to mark arthropods for dispersal research. Methods for administering internal and external protein marks on arthropods are demonstrated. Protein mark detection techniques, either through indirect or sandwich enzyme-linked immunosorbent assays (ELISAs), are also illustrated.

Generation of Marked and Markerless Mutants in Model Cyanobacterial Species

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Cited by 36 •

2016

Introducing multiple genomic alterations into cyanobacteria is an essential tool in the development of strains for industrial and basic research purposes. We describe a system for generating unmarked mutants in the model cyanobacterial species Synechocystis sp. PCC6803 and marked mutants in Synechococcus sp. PCC7002.

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