Manual Cleaning Time

Manual cleaning time is the duration spent physically removing blood, tissue, and other contaminants from reusable medical devices before disinfection or sterilization. During this step, trained personnel apply a compatible detergent and use actions such as brushing, wiping, or flushing to loosen and remove soil from external surfaces, joints, channels, and other hard-to-reach areas; the required duration depends on the device, contamination, cleaning method, and manufacturer’s instructions. In medicine, controlling manual cleaning time supports effective reprocessing, helps reduce residual bioburden, and protects patients and healthcare workers. Consistent timing also improves quality assurance and supports standardized infection-prevention practices.

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JoVE Science Education - Chemistry

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2023

Source: Vy M. Dong and Daniel Kim, Department of Chemistry, University of California, Irvine, CA Organic synthesis is about transforming a readily available reagent into a more valuable product. Having clean glassware is crucial for the efficiency of this process. Dirty glassware can potentially affect the reaction and make isolation of the final product more challenging. Thus, a synthetic chemist must keep the glassware spotless. The methods described here will detail different glass cleaning...

Research

JoVE Journal - Biology
Free Sample

Proper Care and Cleaning of the Microscope

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Cited by 2 •

2008

Keeping the microscope optics clean is important for high-quality imaging. Dust, fingerprints, excess immersion oil, or mounting medium on or in a microscope causes reduction in contrast and resolution. DIC is especially sensitive to contamination and scratches on the lens surfaces. This protocol details the procedure for keeping the microscope clean.

Cleaning, Sterilization, and Disinfection

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2023

Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease. Cleaning The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...

Rabbit Handling and Manual Restraint

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2026

All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Rabbits possess powerful hindlimb musculature and are susceptible to spinal injury if they struggle or kick while inadequately supported. Because rabbits vary in temperament and may struggle during restraint, handlers should...

Selective Cleaning of Caenorhabditis Worms for Enrichment of Intestinal Microbiota

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2026

Source: Morgan, E., et al. Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria. J. Vis. Exp. (2021)This video demonstrates a protocol for isolating intestinal bacteria of interest from wild Caenorhabditis worms. The worms are first starved and washed to eliminate residual feeding bacteria from their surface and intestinal lumen while preserving the target microbes. After surface cleaning and multiple crawling steps, the worms are dissected to extract...

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