Mechanical Plaque Control

Mechanical plaque control is the physical removal of dental plaque, a microbial biofilm that accumulates on tooth surfaces and near the gingiva, making it central to oral disease prevention. Toothbrushing and interdental cleaning apply friction or contact to disrupt and dislodge biofilm before it matures, while technique, frequency, and access to proximal surfaces influence effectiveness. In medicine and dentistry, effective plaque control helps reduce gingival inflammation and lowers the risk of dental caries and periodontal disease. It also supports preventive care, patient education, and long-term oral health, particularly for individuals with orthodontic appliances, limited dexterity, or increased disease susceptibility.

Mechanical Plaque Control - Related Videos

Education

JoVE Science Education - Advanced Biology

Plaque Assay: A Method to Determine Viral Titer as Plaque Forming Units (PFU)

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2023

Source: Tilde Andersson1, Rolf Lood1 1 Department of Clinical Sciences Lund, Division of Infection Medicine, Biomedical Center, Lund University, 221 00 Lund, Sweden Viruses that infect prokaryotic organisms, called bacteriophages or simply phages, were identified in the early 20th century by Twort (1) and d'Hérelle (2) independently. Phages have since been widely recognized for their therapeutic value (3) and their influence on human (4), as well as global, ecosystems (5). Current concerns have...

Research

JoVE Journal - Immunology and Infection
Free Sample

Plaque Assay for Murine Norovirus

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Cited by 99 •

2012

Here we describe a method to quantify infectious particles of murine norovirus (MNV), which is the only norovirus that efficiently replicates in cell culture. The plaque assay takes advantage of MNV’s tropism for murine macrophages and can be adapted for use with biological or environmental samples containing MNV.

Quantification of Pseudovirus Particles by Plaque Assay

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2026

Source: Jamieson, T. R., et al., Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection. J. Vis. Exp. (2021)This video describes the virus plaque assay using coronavirus pseudovirus particles. The diluted pseudovirus is added to a monolayer of mammalian cells, followed by incubation with a methylcellulose overlay. Plaques formed by infected cells are counted visually to determine the infectious virus titer.

Evaluation of a Liquid Overlay System for Viral Plaque Assays

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2026

Source: Baer, A. and Kehn-Hall, K. Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems. J. Vis. Exp. (2014).This video demonstrates the use of a liquid microcrystalline cellulose-based overlay in a plaque-based assay by comparing its effectiveness in viral plaque formation with that of a traditional semi-solid agarose overlay.

Assessing Rotavirus Growth Curve by Plaque Assay

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2026

Source: Kadoya, S., Sano, D. Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus. J. Vis. Exp. (2019).This video demonstrates the use of a plaque assay to assess the rotavirus growth curve. The method involves infecting host cells with diluted virus, overlaying with agarose to restrict spread, and staining to visualize plaques formed by viral replication. By counting plaque-forming units at defined time points, the resulting growth curve reveals the lag, exponential, and...

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