Perivascular Flow Probe
Perivascular Flow Probe - Related Videos
09:05
A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells
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Cited by 4 •
2017
Here we present a novel clinical grade isolation and culture method for kidney Perivascular Stromal Cells (kPSCs) based on whole organ perfusion with digestive enzymes and NG2-cell enrichment. With this method, it is possible to acquire sufficient cell numbers for cellular therapy.
08:15
Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue
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Cited by 7 •
2016
Human cardiac tissue harbours multipotent perivascular precursor cell populations that may be suitable for myocardial regeneration. The technique described here allows for the simultaneous isolation and purification of two multipotent stromal cell populations associated with native blood vessels, i.e. CD146+CD34- pericytes and CD34+CD146- adventitial cells, from the human myocardium.
02:22
Positioning Laser Doppler Probe: A Technique to Implant Probe Over Middle Cerebral Artery for Blood Flow Measurement in Murine Model
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2025
This video describes the surgical procedure to immobilize the laser Doppler probe over the murine brain's middle cerebral artery, or MCA. The probe helps to measure the blood flow in the brain's microvasculature by measuring the Doppler shift caused due to the motion of red blood cells within the artery.
07:05
Use of Human Perivascular Stem Cells for Bone Regeneration
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Cited by 42 •
2012
Human perivascular stem cells (PSCs) are a novel stem cell class for skeletal tissue regeneration similar to mesenchymal stem cells (MSCs). PSCs can be isolated by FACS (fluorescence activated cell sorting) from adipose tissue procured during standard liposuction procedures, then combined with an osteoinductive scaffold to achieve bone formation in vivo.
10:16
Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism (Microcystis aeruginosa) Using Flow Cytometry
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Cited by 3 •
2016
Microbial populations contain substantial cell heterogeneity, which can dictate overall behavior. Molecular probe analysis through flow cytometry can determine physiological states of cells, however its application varies between species. This study provides a protocol to accurately determine cell mortality within a cyanobacterium population, without underestimating or recording false positive results.
FAQs
The two directions provide a comparison rather than a single transit-time measurement. Blood movement changes how quickly sound travels along each path, so the difference between upstream and downstream transit times reflects the velocity of flow. This directional comparison allows the probe to capture blood movement through the vessel in a way that supports hemodynamic assessment.
Transit-time differences provide information about how fast blood moves and the volume moving through the vessel. Velocity describes the movement of blood, while flow volume expresses the amount passing through the vessel. Considering both aspects helps researchers evaluate changing blood-flow dynamics rather than relying only on a single instantaneous observation.
Real-time monitoring allows blood-flow changes to be observed as physiological conditions change. This makes it possible to associate altered flow with cardiovascular function and hemodynamics while an experiment, operation, or vascular intervention is occurring. Continuous observations can therefore show how the circulation responds over time instead of providing only a fixed measurement.
The probe is positioned around the blood vessel, and its paired transducers send sound pulses in upstream and downstream directions. The system compares the transit times of those pulses, then uses their difference to assess blood-flow velocity and volume. Researchers can monitor the resulting measurements while studying arterial or venous flow.
This approach is used during physiological experiments, surgery, and evaluation of vascular therapies. In each setting, monitoring provides a way to observe arterial or venous flow while the relevant physiological condition or intervention is being examined. The measurements can help determine how a procedure or therapy affects cardiovascular function and local hemodynamics.
Flow measurements help connect blood-flow dynamics with tissue perfusion, organ function, and cardiovascular disease. Changes recorded in a vessel can therefore be considered alongside the condition of the tissues or organs it supports. This relationship gives medical researchers a way to study how vascular behavior relates to broader physiological performance and disease-related changes.