Rat Vein Graft
Rat Vein Graft - Related Videos
15:21
Training a Sophisticated Microsurgical Technique: Interposition of External Jugular Vein Graft in the Common Carotid Artery in Rats
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Cited by 9 •
2012
Neointimal hyperplasia is the primary cause of stenosis in arterialized veins. We propose a new protocol whereby the right external jugular vein is grafted using the cuff technique in the common carotid artery of Sprague Dawley rats. The survival rate was 100 % at the time point of sacrifice.
03:42
Jugular Vein Graft Harvest From a Rabbit Model: A Surgical Procedure to Excise a Segment of Rabbit Jugular Vein for Ex Vivo Studies
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2025
In this video, we demonstrate a procedure for surgically harvesting a segment of the jugular vein from a rabbit model. The resected segment can be used as an autologous vein graft to study intimal hyperplasia that develops due to arterial blood pressure.
07:11
Sampling Blood from the Lateral Tail Vein of the Rat
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Cited by 82 •
2015
Blood samples are useful for assessing biomarkers of physiological states or disease in vivo. Here we describe the methodology to sample blood from the lateral tail vein in the rat. This method provides rapid samples with minimal pain and invasiveness.
05:27
Drawing Blood from Rats through the Saphenous Vein and by Cardiac Puncture
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Cited by 87 •
2007
Blood draws are necessary in a large number of studies, for example to study the pharmacokinetics profile of a compound. Here, we demonstrate how to draw blood from rats using two techniques: blood draw from the saphenous vein or by cardiac puncture.
05:09
Urethral Stricture Induction Followed by Buccal Mucosa Graft Urethroplasty in a Rat Model
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Cited by 3 •
2023
In the present protocol, a urethral stricture induction was developed in Wistar rats, followed by urethral reconstruction with a buccal mucosa graft. A retrograde urethrogram and laser Doppler assessment were performed, validating urethral reconstruction (after stricture formation) and graft placement.
FAQs
The arterial environment exposes the implanted vein to altered pressure and flow compared with its original setting. These hemodynamic changes can act alongside endothelial injury and inflammation to initiate cellular and matrix responses. Following this progression helps researchers connect the graft's changing environment with vascular remodeling and later lumen narrowing.
Endothelial injury and inflammation provide early biological signals that can promote smooth-muscle-cell migration and proliferation. These cells also contribute to extracellular-matrix deposition, changing the structure of the graft wall. Studying these linked responses helps investigators examine how an initially injured vessel develops intimal hyperplasia and may progress toward stenosis.
Neointimal formation adds tissue within the graft lumen as smooth-muscle activity and extracellular-matrix deposition progress. This inward growth can progressively reduce the available passageway, producing stenosis. Because the model can also be used to study thrombosis and graft failure, researchers can evaluate several related outcomes of vascular remodeling rather than focusing on a single cellular event.
The central workflow is to implant a vein segment into the rat's arterial circulation, allow the graft response to develop, and then examine remodeling or failure-related outcomes. Researchers can use this controlled sequence to investigate intimal hyperplasia, thrombosis, stenosis, and other responses associated with vascular intervention.
Researchers use the model to compare surgical techniques, biomaterials, drug treatments, and molecular targets in a setting where vascular remodeling can be examined. Outcomes such as graft narrowing, thrombosis, or failure provide measurable evidence of how an intervention influences the biological response. This supports testing strategies intended to modify adverse vascular changes.
The model reproduces several biological processes relevant to human vascular disease, including intimal hyperplasia, thrombosis, stenosis, and graft failure. Its reproducibility and accessibility make it useful for controlled studies of vascular interventions. Findings can therefore provide experimental context for understanding disease mechanisms and evaluating approaches before broader translational consideration.