Adenoviral Cre
Adenoviral Cre - Related Videos
03:20
Large-Scale Production of High-Capacity Adenoviral Vectors
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2026
Source: Ehrke-Schulz, E., et al. Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5. J. Vis. Exp. (2016)This video demonstrates the large-scale amplification of high-capacity adenoviral vectors in suspension-adapted human embryonic kidney cells expressing Cre recombinase for use in gene delivery applications requiring high-titer, helper-free vector preparations.
05:19
Segmental Adeno-Cre Infection: A Technique to Generate Isolated Colorectal Cancer using Genetically Engineered Mouse Models
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2023
This video describes the generation of a colorectal mouse model by segmental adeno-cre infection. The infected colon segment leads to tumorigenesis within this segment of the mucosa, resulting in adenomas ultimately progressing to invasive and metastatic carcinoma. This model is an attractive platform for studies of cancer biology and preclinical therapeutic trials.
15:33
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
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Cited by 7 •
2013
Adenoviral gene transfer into naive CD4 T cells with transgenic expression of the Coxsackie adenovirus receptor enables the molecular analysis of regulatory T cell differentiation in vitro.
04:35
Cesium Chloride-Based Purification of Genome-Containing Adenoviral Vectors
0 Views •
2026
Source: Ehrke-Schulz, E., et al. Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5. J. Vis. Exp. (2016)This video demonstrates the purification of high-capacity adenoviral vectors using cesium chloride gradient ultracentrifugation, enabling the separation of genome-containing virions for applications in gene delivery, vector characterization, and preclinical therapeutic...
02:45
Assessment of Viral Fusion Inhibitors Using a Cre-Inducible Fluorescent Reporter
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2026
Source: Esposito, A. M.,et al. High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion. J. Vis. Exp. (2018)This video demonstrates a co-culture fluorescent assay to monitor cell-to-cell viral transmission. The assay uses a red-to-green reporter cassette to visualize viral membrane fusion and evaluate the efficacy of test compounds in blocking viral entry.
FAQs
After infection, Cre recombinase identifies paired loxP sites within the engineered allele and catalyzes recombination between them. This reaction changes the DNA segment located between the sites, rather than altering unrelated genomic regions. The resulting site-specific change allows researchers to control a gene according to the placement and design of its loxP sequences.
The outcome depends on how the target allele has been engineered around its loxP sites. Recombination can excise the intervening sequence, rearrange it, or produce a configuration that activates or deletes a gene. Thus, the same Cre-delivery principle supports different experimental purposes, provided the allele design matches the intended genetic manipulation.
Transient expression limits Cre activity to a defined experimental window instead of requiring continuous production of the recombinase. Combined with localized delivery, this can provide spatial and temporal control over when and where a conditional allele changes. That flexibility helps investigators test gene function in selected brain regions without relying on a permanently active Cre source.
Recombination is shaped by the cells that receive the recombinant adenovirus and by the brain region selected for delivery. It also requires a compatible engineered allele containing the relevant loxP sites. These factors connect viral targeting with allele design, enabling manipulation in defined cell populations or anatomical areas rather than across the entire nervous system.
Researchers first use a conditional allele containing appropriately positioned loxP sites, then deliver recombinant adenovirus to the selected brain region or cell population. Infection introduces Cre into the target cells, where recombination modifies the allele. The resulting gene activation, deletion, or rearrangement can then support analysis of the chosen neuroscience question.
Adenoviral Cre can support studies of neuronal development, circuit function, behavior, and disease mechanisms by changing gene activity in selected neural locations or cell populations. This localized strategy links a genetic manipulation to a defined part of the nervous system, helping researchers examine how the affected gene contributes to regional or cellular phenotypes.