Cell Culture Inserts
Cell Culture Inserts - Related Videos
11:29
Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact
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Cited by 31 •
2016
In multicellular organisms, secreted soluble factors elicit responses from different cell types as a result of paracrine signaling. Insert co-culture systems offer a simple way to assess the changes mediated by secreted soluble factors in the absence of cell-cell contact.
09:23
A Rapid Filter Insert-based 3D Culture System for Primary Prostate Cell Differentiation
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Cited by 7 •
2017
Here, we present a method for the establishment of a rapid in vitro system that supports the three dimensional culturing and subsequent luminal differentiation of primary prostate epithelial cells.
11:56
Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures
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Cited by 1 •
2010
Fabrication and validation of an add-on platform that offers enhanced control over the spatial and temporal oxygenation in a 6-well plate. The device is adaptable to a number of culture systems and can be used to investigate the effects of oxygen on wound healing.
12:58
Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons
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Cited by 8 •
2012
By tagging the extracellular domains of membrane receptors with superecliptic pHluorin, and by imaging these fusion receptors in cultured mouse neurons, we can directly visualize individual vesicular insertion events of the receptors to the plasma membrane. This technique will be instrumental in elucidating the molecular mechanisms governing receptor insertion to the plasma membrane.
08:17
An Innovative 3D-Printed Insert Designed to Enable Straightforward 2D and 3D Cell Cultures
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2023
In this paper, a newly designed 3D-printed insert is presented as a model of co-culture and validated through the study of the paracrine intercellular communication between endothelial cells and keratinocytes.
FAQs
The porous membrane allows nutrients, signaling molecules, and experimental compounds to move between compartments without mixing the cell populations themselves. This arrangement lets researchers examine communication driven by soluble factors while preserving separation between the interacting cells. In neuroscience, that distinction helps clarify how neurons, glia, or endothelial cells influence one another under controlled exposure conditions.
Physical separation allows researchers to investigate cellular signaling without requiring direct contact between the populations. Because the compartments remain distinct, investigators can relate observed responses to substances that cross the membrane or to deliberately controlled exposure conditions. This is useful for examining communication among neurons, glial cells, and brain endothelial cells in defined in vitro models.
Researchers can vary which cell populations occupy the separate compartments and can manipulate the exposure of one or both populations to experimental compounds. The membrane then provides a controlled route for diffusion between them. Such arrangements support comparisons of how neural, glial, or endothelial cells respond to signaling molecules, transport processes, inflammation-related conditions, or potentially neurotoxic exposures.
A typical setup places selected cells on the porous membrane within a culture well and establishes a second cellular or exposure compartment on the opposite side. Researchers then maintain the separated populations while controlling what each compartment receives. Measurements can focus on intercellular signaling, compound movement, or cell responses under the chosen culture and exposure conditions.
These systems can position brain endothelial cells in relation to another cellular compartment, creating a simplified model for examining barrier-associated transport and signaling. The membrane permits diffusion while maintaining population separation, allowing researchers to study how substances or cellular signals move across the model. This approach provides defined experimental control without requiring a more complex tissue model.
Cell Culture Inserts support co-culture investigations of neural signaling, inflammation, and neurotoxicity involving neurons, glial cells, and brain endothelial cells. Researchers can assess how one population affects another through diffusible signals or how experimental compounds influence separated compartments. The resulting observations help connect cellular communication and transport processes with broader nervous-system responses in vitro.