Infrared Pupillometer
Infrared Pupillometer - Related Videos
08:11
Infrared Spectroscopy
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2023
Source: Vy M. Dong and Zhiwei Chen, Department of Chemistry, University of California, Irvine, CA This experiment will demonstrate the use of infrared (IR) spectroscopy (also known as vibrational spectroscopy) to elucidate the identity of an unknown compound by identifying the functional group(s) present. IR spectra will be obtained on an IR spectrometer using the attenuated total reflection (ATR) sampling technique with a neat sample of the unknown.
09:38
Characterizing Far-infrared Laser Emissions and the Measurement of Their Frequencies
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Cited by 4 •
2015
We describe the generation of far-infrared radiation using an optically pumped molecular laser along with the measurement of their frequencies with heterodyne techniques. The experimental system and techniques are demonstrated using difluoromethane (CH2F2) as the laser medium whose results include three new laser emissions and eight measured laser frequencies.
10:12
Near-infrared Fluorescence Imaging of Abdominal Aortic Aneurysms
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2023
Source: Arvin H. Soepriatna1, Kelsey A. Bullens2, and Craig J. Goergen1 1 Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 2 Department of Biochemistry, Purdue University, West Lafayette, Indiana Near-infrared fluorescence (NIRF) imaging is an exciting optical technique that utilizes fluorescent probes to visualize complex biomolecular assemblies in tissues. NIRF imaging has many advantages over conventional imaging methods for noninvasive imaging of diseases.
01:45
Visualization of Mouse Cerebral Thrombi Using Near-Infrared Fluorescence Imaging
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2025
Source: Kim, D. E., et al. Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli. J. Vis. Exp. (2016)This video demonstrates near-infrared fluorescent (NIRF) imaging to visualize thrombi in excised brain tissue labeled with a fibrin-specific probe. Near-infrared light excites the probe, causing the thrombus to fluoresce, enabling high-contrast thrombi visualization in the major cerebral artery and small cortical blood vessels.
12:27
Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging
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Cited by 3 •
2009
The assembly of a nearfield infrared microscope for imaging protein aggregates is described.
FAQs
Infrared illumination allows the instrument’s camera to follow the pupil while the measured system is exposed to controlled or changing light conditions. The resulting image sequence supports software-based measurement of diameter over time, including constriction, dilation, latency, and recovery. This combination makes the recording objective and suitable for rapid, repeatable assessment.
These response features describe different parts of the pupil’s reaction to a light condition. Constriction and dilation capture the direction of change, latency indicates timing, and recovery captures the return phase. Taken together, they provide a more detailed profile than a single diameter value and can serve as objective indices of autonomic and neural function.
Controlled light conditions allow responses to be characterized under a defined stimulus, whereas changing light conditions show how the pupil responds to altered illumination. Using either design, or comparing responses across conditions, helps investigators examine the pupillary light reflex and evaluate effects associated with pharmacological or neurological perturbations.
A basic workflow is to apply a controlled or changing light condition, record pupil images with infrared illumination and a camera, and use software to quantify the response over time. The analysis can extract diameter, constriction, dilation, latency, and recovery. Because recordings are rapid and repeatable, the same workflow can support measurements across experimental conditions.
In neuroscience, infrared pupillometry can be used to study the pupillary light reflex, arousal, and attention, and to characterize responses to pharmacological or neurological perturbations. It also supports investigations of autonomic and neural dysfunction. These applications benefit from objective measurements that capture response timing and diameter changes rather than relying only on observational judgment.
Rapid, repeatable recordings allow investigators to compare pupil responses across controlled conditions or across perturbations. Differences in constriction, dilation, latency, or recovery can then be examined as changes in autonomic or neural function. This is particularly useful when a study needs a consistent outcome measure for characterizing dysfunction or evaluating responses over repeated assessments.