Intracerebral Hemorrhage Model
Intracerebral Hemorrhage Model - Related Videos
10:44
Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase
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Cited by 60 •
2012
Clinically relevant animal models of intracerebral hemorrhage (ICH) are needed to extend our knowledge of hemorrhagic stroke and to examine novel therapeutic strategies. In this study, we describe and evaluate two ICH models that implement unilateral injections of either autologous whole blood or bacterial collagenase into the basal ganglia (corpus striatum) of mice.
07:02
Autologous Blood Injection to Model Spontaneous Intracerebral Hemorrhage in Mice
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Cited by 28 •
2011
The autologous blood injection model of intracerebral hemorrhage in mice described in this protocol uses the double injection technique to minimize risk of blood reflux up the needle track, no anticoagulants in the pumping system, and eliminates all dead space and expandable tubing in the system.
09:41
Intrastriatal Injection of Autologous Blood or Clostridial Collagenase as Murine Models of Intracerebral Hemorrhage
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Cited by 35 •
2014
Preclinical models of intracerebral hemorrhage are utilized to mimic certain aspects of clinical disease. Thus, mechanisms of injury and potential therapeutic strategies may be explored. In this protocol, two models of intracerebral hemorrhage are described, intrastriatal (basal ganglia) injections of autologous blood or collagenase.
09:01
Minimally Invasive Endoscopic Intracerebral Hemorrhage Evacuation
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Cited by 3 •
2021
This paper details the surgical protocol for minimally invasive endoscopic intracerebral hemorrhage evacuation using the SCUBA technique.
04:35
Establishing a Subarachnoid Hemorrhage Rat Model Induced by Autologous Blood Injection
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2025
This video demonstrates the procedure for establishing a subarachnoid hemorrhage rat model. An anesthetized rat is implanted with probes to measure intracranial pressure and cerebral blood flow. The target site is identified, and the needle is inserted at a pre-chiasmatic position. Autologous blood is then injected into the subarachnoid space, increasing intracranial pressure and decreasing cerebral blood flow, establishing the model.
FAQs
The hematoma disrupts surrounding neural tissue and initiates several linked responses, including blood-brain barrier breakdown, edema, inflammation, and neuronal injury. These secondary changes extend the biological consequences beyond the initial bleeding event. Measuring them helps researchers distinguish lesion formation from later tissue responses and examine how hemorrhagic injury progresses within the brain.
Autologous blood and collagenase provide two established ways to create a hematoma in the brain. Using either approach gives investigators a controlled starting point for examining lesion development, neurological deficits, cellular responses, and recovery. The choice of induction approach therefore forms part of the experimental design when studying how hemorrhagic injury evolves over time.
Researchers can assess several complementary outcomes, including lesion development, neurological deficits, cellular responses, and recovery over time. Together, these measurements connect structural changes in the injured brain with functional consequences and evolving biological reactions. This longitudinal perspective is important because hemorrhagic injury includes both the initial lesion and subsequent responses in neural tissue.
A typical workflow uses a rodent brain and introduces either autologous blood or collagenase through an injection designed to produce a hematoma. Investigators then monitor the resulting lesion and evaluate neurological deficits, cellular responses, and recovery. This sequence provides a defined experimental injury followed by outcome measurements that can be compared across treatment or research conditions.
The model is used when investigators need a controlled platform for testing approaches to hemorrhagic brain injury. Supported applications include evaluating neuroprotective treatments, surgical strategies, and rehabilitation approaches. Researchers can judge these interventions by examining effects on lesion development, neurological deficits, cellular responses, or recovery, allowing each strategy to be studied against measurable consequences of the hemorrhage.
In neuroscience, the system links a defined intracerebral bleed with changes in neural tissue, barrier integrity, edema, inflammation, neuronal injury, and behaviorally relevant neurological deficits. Because researchers can follow these outcomes over time, the model supports investigation of hemorrhage mechanisms as well as recovery. It also provides a structured setting for comparing therapeutic and rehabilitation strategies.