Phase Image Analysis
Phase Image Analysis - Related Videos
04:37
Three-Dimensional Quantitative Phase Imaging for Characterizing Lymphocyte Subtypes
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2025
This video demonstrates label-free identification of lymphocyte morphology and biochemistry through 3D quantitative phase imaging. This technique allows for a thorough analysis of lymphocytes, uncovering their internal structures and properties without the need for labeling procedures.
11:24
High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
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Cited by 1 •
2025
The combination of iterative-bleaching-extends-multiplexity (IBEX) and a commercial nucleotide labeling assay (Click-iT EdU) enables the detection and categorization of dividing cell types in highly dynamic processes in fixed frozen murine tissue sections. Furthermore, a novel open-source image processing pipeline provides high-throughput image acquisition and analysis.
07:02
Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery
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Cited by 5 •
2018
The overall goal of this procedure is to measure the blood flow in the rat common carotid artery by using noninvasive phase contrast magnetic resonance imaging.
13:45
Hybrid µCT-FMT imaging and image analysis
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Cited by 34 •
2015
We describe a protocol for hybrid imaging, combining fluorescence-mediated tomography (FMT) with micro computed tomography (µCT). After fusion and reconstruction, we perform interactive organ segmentation to extract quantitative measurements of the fluorescence distribution.
08:02
Reservoir Condition Pore-scale Imaging of Multiple Fluid Phases Using X-ray Microtomography
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Cited by 28 •
2015
We present a methodology for the imaging of multiple fluid phases at reservoir conditions by the use of x-ray microtomography. We show some representative results of capillary trapping in a carbonate rock sample.
FAQs
The measured signal reflects optical path length, which changes when light travels through regions with different cell thicknesses or refractive indices. A thicker region or a region with different optical properties can therefore produce a distinct phase or image-intensity value. Interpreting these variations allows researchers to relate image data to structural differences within transparent neural cells.
Phase or intensity differences provide measurable image values rather than only visual contrast. Computational analysis can use those values to assess features such as neuronal morphology, growth, viability, and cellular dynamics. This quantitative approach is especially useful when structural or behavioral changes develop over time in cultured neurons or other neural cells.
Its major distinction is that it can monitor living samples without fluorescent labels, whereas molecular imaging commonly relies on labeled targets. This makes the approach complementary rather than a replacement for fluorescence. Researchers can follow cellular structure and behavior over time with phase information, then combine those observations with molecular measurements when biological identity or pathway-specific information is needed.
Changes in image intensity or phase can reveal shifts associated with neuronal morphology, growth, viability, and cellular dynamics. These measurements support analysis of how neural cells change rather than providing only a single endpoint observation. In practice, the resulting quantitative data can help characterize development, disease-related changes, or responses to treatment in cultured neural models.
A typical workflow begins by recording phase-contrast or related microscopy images from transparent neural cells. The resulting phase or intensity information is then analyzed computationally to extract measurable cellular features. Repeated imaging can support longitudinal assessment, allowing morphology, growth, viability, or dynamics to be compared across developmental conditions, disease models, or treatment responses.
Phase Image Analysis is useful when researchers need to observe living neural samples repeatedly during development or experimental treatment. Because the measurements do not require fluorescent labels, the same cultured neurons or other neural cells can be monitored for changes in morphology, growth, viability, and dynamics. This supports time-dependent comparisons in disease models and treatment-response studies.
Phase imaging supplies quantitative information about cellular structure and behavior, while molecular imaging can provide information associated with labeled biological targets. Using both approaches can connect visible changes in neuronal morphology or dynamics with molecular observations. In neural development, disease models, and treatment experiments, this complementary strategy broadens interpretation beyond either image type alone.