Saline Perfusion
Saline Perfusion - Related Videos
03:15
A Hypertonic Saline Injection Technique for Inducing Glaucoma in Rats
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2025
This video demonstrates glaucoma induction in an anesthetized rat by injecting hypertonic saline into the episcleral vein. The saline enters the trabecular meshwork via Schlemm's canal, causing tissue scarring, which blocks aqueous humor outflow and raises intraocular pressure. The elevated pressure damages retinal ganglion cells, leading to glaucoma.
01:17
Salinity Tolerance of Alishewanella Species Isolated from Seawall Muddy Water
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2025
Begin with a culture of Alishewanella, a Gram-negative, salt-tolerant bacterium.Next, streak the culture onto agar plates containing increasing concentrations of sodium chloride to simulate varying levels of saline stress.Overlay each plate with a layer of unsolidified medium to prevent oxygen diffusion and maintain the culture in an oxygen-limited environment.Seal and incubate the plates at an optimum temperature to support bacterial growth.During incubation, the bacterium exhibits varying...
06:29
Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs
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Cited by 2 •
2016
Embryonic stages are the most susceptible to xenobiotics. Although chemical toxicity depends on salinity, no method exists to test the salinity dependence of toxicity to aquatic organisms. Here, we describe a new and high-throughput method for determining the salinity dependence of toxicity to aquatic embryos.
04:44
Antegrade Perfusion Model: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
This video describes an antegrade perfusion technique of an isolated mouse heart during which a perfusate is injected through the left ventricle, and eventually, it perfuses the entire myocardium. This method can be used to isolate cardiomyocytes from murine models and generate decellularized hearts for further downstream applications.
12:13
Ex Situ Normothermic Machine Perfusion of Donor Livers
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Cited by 21 •
2015
Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.
FAQs
An isotonic solution helps replace blood within the vascular system while maintaining a solution environment compatible with the tissue during preparation. This supports more consistent removal of blood from cerebral and peripheral vessels than a preparation that leaves substantial blood behind. The result is cleaner tissue for subsequent fixation, staining, and microscopic examination.
Residual blood can interfere with tissue preservation, staining, and imaging by adding blood-derived background to the sample. In brain and spinal cord sections, that background may make neural structures or cellular markers harder to evaluate clearly. Removing blood before collection helps researchers distinguish tissue-associated signals from material remaining in the vasculature.
Replacing blood throughout the vascular system improves the consistency of the collected tissue by reducing residual blood in both cerebral and peripheral vessels. This matters because uneven blood removal can contribute to differences in sample appearance and background. More consistent preparation supports clearer comparisons of neural structures, cellular markers, and disease-related changes.
Saline perfusion is performed as a preparation step before tissue collection or fixation. The solution is delivered through the heart so it can circulate through the animal’s vascular system and displace blood before the brain, spinal cord, or other tissues are processed. This sequence prepares cleaner samples for later histological, immunohistochemical, or anatomical analysis.
The method is particularly useful when researchers need clear tissue for histology, immunohistochemistry, or anatomical analysis. Reduced blood-related background can improve the visibility of neural structures and cellular markers during staining and imaging. It therefore supports studies that measure tissue organization, marker distribution, or disease-related changes in the brain and spinal cord.
Prepared samples can support clearer evaluation of neural structures, cellular markers, and disease-related changes. Because less residual blood remains in the vessels, staining and imaging are less affected by blood-derived background. This improved clarity helps researchers obtain more interpretable anatomical and cellular measurements from brain, spinal cord, and related tissue samples.