Spinal Needle Guidance
Spinal Needle Guidance - Related Videos
10:30
Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
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Cited by 10 •
2014
We describe here the use of a pH-sensitive green fluorescent protein variant, pHluorin, to study the spatio-temporal dynamics of axon guidance receptors trafficking at the cell surface. The pHluorin-tagged receptor is expressed both in cell culture and in vivo, using electroporation of the chick embryo.
02:58
Laser Doppler Needle Probe Insertion: A Technique for Real-time Quantification of Blood Flow within the Spinal Cord in a Porcine Model
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2025
This video demonstrates the placement of a laser Doppler needle probe in the spinal cord of a porcine model. This helps in the continuous quantification of blood supply to the spinal cord, an important assessment in spinal cord injury.
10:14
Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells
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Cited by 9 •
2016
This protocol describes a bioprinting methodology using an automated robotic depositing system that incorporates etched topographical guidance cues with the precision deposition of a cell bearing hydrogel bioink. The printed cells are directly delivered to the etched features and are able to sense and orientate with them.
12:23
Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
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Cited by 41 •
2010
This video demonstrates a method to dissect and culture commissural neurons from E13 rat dorsal spinal cord. Dissociated commissural neurons are useful to study the cellular and molecular mechanisms of axon growth and guidance.
09:51
Intraosseous Needle Placement
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2023
Source: Julianna Jung, MD, FACEP, Associate Professor of Emergency Medicine, The Johns Hopkins University School of Medicine, Maryland, USA For unstable patients requiring urgent administration of medications, fluids, or blood products, establishing vascular access quickly is essential. However, there are many factors that can complicate placement of a peripheral intravenous cannula (PIV), and it is extremely common for PIV attempts to fail. PIV placement may be technically challenging in small...
FAQs
Anatomical landmarks help identify the intended entry site within the spinal region before needle insertion. Their value lies in translating visible or palpable structural features into a planned access point, giving the operator a consistent starting reference. This can support repeatable positioning across neuroscience procedures when imaging or another localization method is not being used.
A controlled trajectory helps keep the needle aligned with the intended path after the entry site has been identified. This approach limits unnecessary tissue contact and supports more consistent access to the target. In neuroscience experiments, improved trajectory control can influence delivery performance, sampling consistency, and the reliability of comparisons between procedures.
Imaging and other localization methods provide alternatives or additional guidance when anatomical landmarks alone do not supply the desired localization information. Each approach can help identify the entry site and support alignment toward the intended target. Selecting among these options matters because accurate positioning contributes to procedural reproducibility and consistent interpretation of results.
A basic workflow begins by identifying the intended spinal entry site with anatomical landmarks, imaging, or another localization method. The needle is then positioned and advanced along a controlled trajectory toward the target. Maintaining this planned alignment throughout the procedure helps reduce unnecessary tissue contact and supports consistent access for sampling or delivery.
In neuroscience, spinal needle guidance supports procedures that require access to the spinal region, including cerebrospinal fluid sampling, intrathecal delivery, and experimental access to neural structures. The guidance approach is especially relevant when placement accuracy can affect the quality of a collected sample, the performance of a delivered substance, or the interpretation of experimental findings.
Accurate placement can affect several downstream outcomes rather than serving only as a technical goal. For cerebrospinal fluid sampling, it may influence sample quality; for intrathecal delivery, it may affect delivery performance; and for experimental neural access, it can support more consistent procedures. These effects help researchers interpret results in relation to the intended target.