Oxytocin

Oxytocin is a peptide hormone and neuropeptide that coordinates reproductive physiology while influencing social behavior, emotional processing, and attachment. Produced in the hypothalamus and released through the posterior pituitary and neural pathways, it acts on oxytocin receptors to support uterine contractions, milk ejection, and context-dependent responses to social cues. In psychology, oxytocin research examines its roles in bonding, trust, stress regulation, caregiving, and interpersonal communication, while recognizing that its effects vary with individual experience and social context. Studying this system helps connect brain mechanisms with human relationships and informs research on development, mental health, and social behavior.

Oxytocin - Related Videos

Research

JoVE EoE - Neuropathology

Isolation of Oxytocin Receptor-Rich Nuclei from a Neonatal Rat Brain

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2025

This video demonstrates the isolation of oxytocin receptor-rich nuclei from the neonatal rat brain. Coronal slicing, followed by identification and precise punching of oxytocin receptor-rich brain nuclei, enables the effective extraction of oxytocin receptor-rich nuclei for further analysis.

Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

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Cited by 7 •

2018

Here, we present a protocol to isolate brain nuclei in the neonatal rat brain in conjunction with first colostrum feeding. This technique allows the study of nutrient insufficiency stress in the brain as modulated by enterocyte signaling.

Evaluation of Mammary Gland Development and Function in Mouse Models

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Cited by 98 •

2011

This method describes how to dissect and assess mammary gland development and function from mice. Excised mammary glands are assessed for the degree of development using whole mount while milk ejection is evaluated using an oxytocin-based myoepithelial cell contraction assay.

Milk Collection Methods for Mice and Reeves' Muntjac Deer

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Cited by 26 •

2014

Milk collection from animal models facilitates various research avenues: understanding passive immunity, identifying pathogens responsible for vertical transmission and, through the use of transgenic mice, even commercial production of proteins found in human breast milk. Here we illustrate a simple method for milk collection in mice and Reeves’ muntjac deer.

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit

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Cited by 24 •

2015

Milk is a primary source of nutrition for the neonate. Analysis of milk components may provide insight into maternal factors that affect offspring health. This protocol describes a manual method of collecting milk samples from the lactating rat, which can then be used for further downstream analysis.

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