Supplication

Supplication is an earnest request for help, support, or intervention, often directed toward another person, group, or perceived higher power. In psychological contexts, it involves communicating need through language, emotion, and appeals to a recipient’s willingness or ability to respond, and may function as a form of help-seeking, coping, or emotion expression. Studying supplication can clarify how people manage distress, seek social or spiritual support, and negotiate dependence, vulnerability, and interpersonal influence. Research may also examine how cultural beliefs, perceived control, and relationship context shape when individuals supplicate and how others respond.

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Research

JoVE Journal - Biology

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures

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Cited by 5 •

2013

Here we describe a robust method for the fractionation of plant plasma membranes into detergent resistant and detergent soluble membranes based on a mixture of unlabeled and in vivo fully 15N labeled Arabidopsis thaliana cell cultures. The procedure is applied for comparative proteomic studies to understand signaling processes.

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