Name: sampling materials in solid, liquid, gas
Uploaded: 2024-12-05T18:31:54.000Z
Duration: 1 min 18 s
Channel: JoVE

Üç tür numune alma malzemesi vardır: katı, sıvı ve gaz. Katı numuneler, su kütlelerinde, kayalarda, metallerde ve biyolojik dokularda biriken tortuları içerebilir.
Su kütlelerindeki tortu, bir kepçe örnekleyici veya bir piston çekirdeği kullanılarak ekstrakte edilir. Örneğin, bir kepçe numune alma cihazını bir nehir yatağına indirdikten sonra, numune alma cihazı bir numune çıkarmak için tortunun etrafını kapatır. 
Sıvı numunelerin bazı örnekleri su, kan, idrar ve çözücülerdir. Homojen sıvının kesin bir hacmi, bir pipet veya şırınga kullanılarak toplanır.
Numuneler, endüstriyel atık sular gibi heterojen sıvılardan, bunları düzenli aralıklarla toplayan otomatik bir numune alıcı kullanılarak elde edilebilir.
Analiz için atmosferik havadan, egzoz deliklerinden ve endüstriyel bacalardan gaz numuneleri toplanabilir.
Aktif karbon gibi katı sorbentler, gaz akışından hedef bileşikleri seçici olarak adsorbe etmek için yaygın olarak kullanılır. Hedef bileşik daha sonra desorpsiyon teknikleri ile ekstrakte edilir ve uygun analitik yöntemler ile analiz edilir.

Örnekleme yöntemleri: Örnek türleri

Örnekleme materyalleri üç ana türe ayrılır. Bunlar katı, sıvı ve gaz’dır.

Katı örnekler, su kütlelerinden alınan tortular, toprak, metaller ve biyolojik dokular gibi çeşitli maddeleri içerir. Su kütlelerinden tortu çıkarmak için kullanılan iki standart yöntem, örnek ve piston korer’dir. Cihaz ile numune alma, minimum bozulma ile bir su kütlesinin dibinden ayrı bir tortu örneği toplamak için bir cihaz kullanmayı içerir. Numuneler, mekansal değişkenlik (farklı konumlardaki değişiklik) nedeniyle her zaman tüm alanı temsil etmez. Piston koreri, tortu katmanları içindeki zaman içindeki değişiklikleri analiz etmeyi sağlayan sürekli bir tortu sütununu almak için kullanılan bir yöntemdir.

Örnek, göller veya nehirler gibi yüzey su kütlelerinden temiz bir örnekleme şişesi kullanılarak toplanabilir. Şişe, kontaminasyonu minimize etmek için istenilen derinlikte suya batırılmalıdır. Kan örneklemesi genellikle bir enjektör kullanılarak, belirli bir kan hacmini almak amacıyla bir damara yerleştirilir. Örnek daha sonra uygun kaplara aktarılır ve ileri analiz için hazırlanır. İdrar örneklemesi, belirli bir idrar hacmini toplamak için temiz bir kap kullanmayı içerir. İdrar örneği, ardından analiz için uygun kaplara aktarılır. Endüstriyel atık sular genellikle çeşitli kimyasallar ve kirleticiler içeren heterojen sıvılardır. Bu tür atık suların örneklenmesi genellikle otomatik bir örnek alma cihazı gerektirir; bu cihaz, düzenli aralıklarla örnek toplamak için tasarlanmış bir cihazdır. Bu cihaz, önceden belirlenen zaman aralıklarında temsili örnekler toplamak üzere programlanmış olup, atık suyun bileşenlerini kapsamlı bir şekilde analiz eder.

Gaz örneklemesi için, hedef bileşenleri bir gaz akışından seçici olarak adsorbe etmek amacıyla genellikle aktif karbon gibi katı sorbentler kullanılır. Aktif karbona adsorbe olan bileşenler, ısıtma ve çözücü ile ekstraksiyon gibi çeşitli desorpsiyon yöntemleriyle çıkarılabilir. Çıkarılan bileşikler, belirli bileşene ve gereken bilgilere bağlı olarak, daha sonra spektroskopi ya da kromatografi gibi karakterizasyon teknikleriyle analiz edilebilir.

Örnekleme Yöntemleri: Örnek Türleri

Numune alma malzemeleri üç ana türe ayrılır: katı, sıvı ve gaz.
Katı numuneler, tortular gibi çeşitli maddeleri içerir ...

Education

JoVE Core

Analytical Chemistry

Analitik Kimya

Unit 1

Ünite 1

Method Development and Sampling Techniques

Yöntem Geliştirme ve Örnekleme Teknikleri

method-development-and-sampling-techniques

KEY TERMS AND CONCEPTS

TEMEL TERİMLER VE KAVRAMLAR

Sampling Methods: Sample Types

sampling materials in solid, liquid, gas

Sampling materials are grouped as solid, liquid, or gas. The type of sample helps decide the right collection method. Solid samples can include sediments from water bodies, soil, metals, and biological tissues.

Sediment sampling from water bodies can be done in two common ways. Grab sampling uses a device to collect a discrete sample from the bottom with minimal disturbance. These samples may not show the full area because conditions can vary from place to place. Piston coring collects a continuous column of sediment. This lets scientists study changes over time in the sediment layers.

Liquid samples are often collected with clean tools to reduce contamination. A sampling bottle can be used for surface water in lakes or rivers. The bottle is submerged to the desired depth before the sample is taken. Blood is usually sampled with a syringe placed into a vein to draw a set volume, then the blood is moved into the correct container for analysis. Urine sampling uses a clean container to collect a measured amount of urine before transfer to suitable containers.

Industrial effluents are often mixed liquids that contain many chemicals and pollutants. They are commonly sampled with an automatic sampler. This device collects samples at set time intervals so the sample better reflects the effluent composition. Gas sampling often uses solid sorbents such as activated carbon. The target compounds are adsorbed onto the carbon, then removed by desorption methods such as heating or solvent extraction. The extracted compounds can then be studied with techniques like spectroscopy or chromatography.

Sampling Materials in Solid, Liquid, Gas

development of analytical methods

Analytical Methods: Technique to Protocol

Analytical methods follow a clear path from technique to protocol. In analytical chemistry, a technique is the scientific principle used to explain a chemical measurement. A method applies that technique to a sample of interest. The next steps are procedure and protocol. A procedure gives the directions for carrying out the analysis with an analytical method. A protocol is the detailed set of guidelines for that procedure, and it should be followed closely to get the desired results. <br...

quality control

Quality Control Charts and Lab Records

Quality control charts, proficiency testing, and lab records are key parts of quality control in a laboratory. These steps help keep a system under statistical control. They also help track data, show trends, and spot variations that may affect the accuracy of an analysis. One common tool is a quality control chart. This chart plots one or more analytical characteristics over time. The measured values are taken at different times during the analysis and compared with the known value of...

quality assurance

Quality Checks in Analytical Testing

Quality assurance in analytical testing brings together the checks that help a system perform properly. It includes quality control, quality assessment, and internal corrective measures. These parts work in a cycle to support reliable results. Quality control happens before and during the analysis. Quality assessment takes place during and after the investigation. Internal corrective measures are then based on the results of post-analysis quality assessment, or they may be applied before a new...

data validation

How Analytical Methods Are Checked

Method validation checks whether an analytical chemistry method gives reliable results. It is especially important when the same method is used with different sample matrices or when the procedure is changed. Validation helps show that the method still meets acceptable standards in each case. Several key checks are used in method validation. Specificity means the method can measure the target analyte without interference from other substances. Linearity means the result changes in direct...

qualitative analysis

Classical and Instrumental Sample ID

Qualitative analysis identifies the elements, ions, or compounds in an unknown sample. It is the first step in the hierarchy of analytical goals. That makes it the starting point for scientific study before quantitative analysis or other forms of analysis. Two main approaches are used in qualitative analysis: classical methods and instrumental methods. Classical methods depend on the visible or sensory result of a chemical reaction, such as a color change, a precipitate, or even a smell. These...

quantitative analysis

Measuring Analyte Concentration

Quantitative analysis measures the amount of a specific substance, called the analyte, in a sample. If the sample composition is unknown, qualitative analysis comes first. That step identifies the components so the correct substances can be measured during the quantitative phase. This method depends on two key measurements. Scientists measure the sample quantity and also a property that is proportional to the amount of analyte. Together, these measurements help determine the analyte...

instrument calibration

Calibrating Lab Instruments for Accurate Results

Calibration helps lab instruments produce accurate and consistent results. It is important in manufacturing, healthcare, testing laboratories, and scientific research. Each instrument needs a calibration process that matches its design and function. This process improves data accuracy and helps results stay reliable. An analytical balance measures mass and needs regular calibration to stay precise. It is calibrated by comparing its readings with known standard weights. These weights act as...

glassware calibration

Calibrating Lab Glassware for Accurate Volume

Calibrating lab glassware helps students measure liquids accurately in the analytical laboratory. It reduces volume errors and supports consistent results across measurements. This matters when using volumetric flasks, pipettes, burettes, and other tools that depend on precise volume readings. Volumetric flasks are used to prepare aqueous solutions with a precise final volume. They have a calibration line on the neck. To calibrate one, fill it with distilled water until the meniscus, the...

standard solutions

Preparing Standard Solutions in the Lab

Standard solutions are prepared to have a precisely known concentration or composition. In analytical chemistry, this accuracy is important because it supports reliable results. A primary standard is a highly pure, stable substance with a high molar mass. It is fully soluble in water, which is the most commonly used solvent in analytical chemistry. A primary standard solution can be used to standardize a secondary standard. Secondary standards have known concentrations, but they are usually...

blank solutions

Blank Solutions in Analytical Testing

Blank solutions are used in analytical testing to show the background signal before a sample is measured. A blank solution does not contain the analyte, which is the substance of interest being tested or measured. It is usually made with the same reagents and procedure as the sample solution, but without the analyte. The main purpose of a blank solution is to control for background interference and contamination. Reagents, solvents, or lab equipment can sometimes create a detectable signal on...

sampling methods: overview

How Sampling Shapes Lab Results

Sampling shapes lab results by determining how a smaller sample represents a larger population. In analytical chemistry, testing an entire population is often impractical or impossible, so samples are collected to make inferences about the whole group. The sampling method is the process used to choose individuals or items for that sample. Common sampling methods include random, judgemental, systematic, stratified, and cluster sampling. Each method can affect the quality of the data in a...

sampling methods: sample types

Sampling materials are grouped as solid, liquid, or gas. The type of sample helps decide the right collection method. Solid samples can include sediments from water bodies, soil, metals, and biological tissues. Sediment sampling from water bodies can be done in two common ways. Grab sampling uses a device to collect a discrete sample from the bottom with minimal disturbance. These samples may not show the full area because conditions can vary from place to place. Piston coring collects a...

sampling plans

Choosing Samples for Reliable Results

Sampling is a key step in analytical chemistry. It helps researchers collect data that represents a larger population. The main sampling methods in this topic are random, judgmental, systematic, stratified, and cluster sampling. Random sampling gives each member of the population an equal chance of being chosen. Researchers often use a random number generator or a lottery-style method. For example, a polymer sheet can be divided into 1 cm x 1 cm sections, and 10 sections can be selected at...

sample preparation for analysis: overview

Preparing Samples by State and Form

Sample preparation depends on the state and form of the sample before analysis. It helps make the sample ready for accurate measurement. The main goal is to extract the analyte, which is the substance being measured, and remove parts that could interfere with the analysis. Solid samples often need the most handling. Large solids are usually ground, crushed, or milled to make them smaller and increase surface area. The sample is then sieved and mixed so it has a uniform composition. After that,...

sample preparation for analysis: advanced techniques

sample-preparation-for-analysis-advanced-techniques

Preparing Complex Samples for Analysis

Preparing complex samples for analysis often requires special digestion and fusion methods. Samples with inorganic or organic material can be hard to dissolve or break down. Common sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion. These steps help turn difficult samples into forms that can be tested reliably and again with similar results. Acid digestion is often used for inorganic materials that do not dissolve in water. It is useful for soil and...

sample handling

Sample Transport and Storage Basics

Sample transport and storage help keep specimens intact before testing. Moving a sample from the collection point to the laboratory requires careful handling. The sample should be sealed well and clearly labeled. These steps help prevent cross-contamination and support reliable results. Temperature control is also important during transport. Some samples travel in insulated containers, cool packs, or refrigerated vehicles so they stay within the needed temperature range. After the samples...

contaminants and errors

Sampling Errors in Sample Preparation

Sampling errors in sample preparation can affect the accuracy of laboratory results. Two common problems are concentration bias from poor sample splitting and contamination from reducing particle size by grinding or homogenization. Recognizing these errors helps make analysis more reliable. The number of samples also matters when a target population follows a normal distribution. In that case, the sample size ns can be found with a formula that uses the t-value for the chosen confidence level,...

key terms and concepts

Explore how solid, liquid, and gas samples are collected, from sediment cores and water bottles to blood, urine, effluents, and activated carbon gas sampling.

Sampling Materials in Solid, Liquid, Gas | JoVE K12

JoVE Core Analitik Kimya, karmaşık kimyasal prensiplerini anlaşılır, ilgi çekici animasyonlara dönüştürerek basitleştirir ve SI Birimlerinden NMR spektroskopisine kadar konuları daha erişilebilir ve anlaşılır hale getirir. Buna ek olarak, bilim insanlarının yer aldığı videolar, bu kavramların dünya çapındaki laboratuvarlarda yürütülen gerçek dünya araştırmalarındaki uygulamalarını gösterir.

analytical chemistry

Sampling Materials in Solid, Liquid, Gas

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