Angiogenic Factors
Angiogenic Factors - Related Videos
10:04
The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro
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Cited by 12 •
2017
Here, we present a novel application of the aortic ring assay where prelabelled mesenchymal cells are co-cultured with rat aorta-derived endothelial networks. This novel method allows visualization of Mesenchymal Stromal Cells (MSCs) homing and integration with endothelial networks, quantification of network properties, and evaluation of MSC immunophenotypes and gene expression.
04:21
Generating Anti-Angiogenic Tumor-Associated Neutrophils
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2025
This video demonstrates an assay to generate anti-angiogenic tumor-associated neutrophils (TANs). Upon taking a murine melanoma tumor containing pro-angiogenic TANs, the single cells are isolated using fluorescence-activated cell sorting (FACS) and treated with an inhibitor of nicotinamide phosphoribosyl transferase (NAMPT) to reduce the capacity to stimulate angiogenesis.
02:16
Transcription Factors
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2019
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
04:01
Factors Affecting Solubility
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2020
Compared with pure water, the solubility of an ionic compound is less in aqueous solutions containing a common ion (one also produced by dissolution of the ionic compound). This is an example of a phenomenon known as the common ion effect, which is a consequence of the law of mass action that may be explained using Le Chȃtelier’s principle. Consider the dissolution of silver iodide: This solubility equilibrium may be shifted left by the addition of either silver or iodide ions, resulting in...
11:07
In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts
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Cited by 3 •
2019
The aim of this method is to generate an in vivo model of tumor angiogenesis by xenografting mammalian tumor cells into a zebrafish embryo that has fluorescently-labelled blood vessels. By imaging the xenograft and associated vessels, a quantitative measurement of the angiogenic response can be obtained.
FAQs
Many angiogenic factors act by binding receptors on endothelial cells, the cells lining blood vessels. Receptor engagement activates signaling pathways that can increase endothelial proliferation, migration, and survival while also promoting extracellular matrix remodeling. Together, these responses alter the local vascular environment and help coordinate vessel growth from existing vasculature.
Local oxygen levels can shift angiogenic signaling, while inflammatory signals add another layer of regulation. The response also depends on communication among blood vessels, stromal cells, and immune cells rather than on a single molecule acting alone. Considering these conditions is essential when interpreting why vascular growth differs between tissues or biological states.
VEGF is a central example because it participates in the signaling that controls endothelial behaviors required for vascular growth. Its effects should therefore be interpreted through cellular responses such as proliferation, migration, survival, and matrix remodeling, rather than as a simple on-or-off switch. This perspective helps connect molecular signals with tissue-level vascular changes.
Researchers can examine whether endothelial cells show increased proliferation, migration, or survival and whether the extracellular matrix undergoes remodeling. They can then interpret those cellular changes alongside local oxygen conditions, inflammatory signals, and interactions with stromal and immune cells. This framework connects molecular activity to the broader process of vascular growth.
During wound healing, angiogenic signaling contributes to the vascular changes needed for tissue repair. Because the response is regulated by oxygen availability, inflammation, and interactions among vascular, stromal, and immune cells, it reflects the local repair environment. Studying these signals can clarify how tissues restore vascular support while maintaining physiological balance.
Their therapeutic relevance depends on whether additional blood-vessel growth is beneficial or harmful. In ischemic tissue, strategies may aim to stimulate angiogenesis, whereas in cancer, treatment may seek to inhibit tumor vascularization. This contrast illustrates why researchers must interpret angiogenic signaling in relation to tissue context, desired outcome, and disease state.