Peritoneal Metastasis Study

A peritoneal metastasis study investigates how cancer cells spread from a primary tumor to the peritoneum, the membrane lining the abdominal cavity, where secondary tumors can impair organ function and worsen prognosis. Researchers examine how tumor cells detach, survive in abdominal fluid, adhere to peritoneal surfaces, and establish metastatic lesions, often using patient samples, imaging, animal models, or laboratory cultures. These studies clarify the cellular and molecular factors that support implantation and progression, helping researchers improve disease staging, identify prognostic biomarkers, evaluate therapies, and develop more effective strategies for preventing or treating advanced abdominal cancers.

Peritoneal Metastasis Study - Related Videos

Research

JoVE Journal - Medicine

Quantitation of Intra-peritoneal Ovarian Cancer Metastasis

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Cited by 15 •

2016

Ovarian cancer metastasis is characterized by numerous diffuse intra-peritoneal lesions, such that accurate visual quantitation of tumor burden is challenging. Herein we describe a method for in situ and ex vivo quantitation of metastatic tumor burden using red fluorescent protein (RFP)-labeled tumor cells and optical imaging.

Modeling Ovarian Cancer Metastasis in Peritoneal Cavity Lining: A Method To Establish an In Vitro 3D Model of the Peritoneal Lining

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2023

In this video, we demonstrate the development of an in vitro three-dimensional model of the lining of the peritoneal cavity. This organotypic culture can help to investigate ovarian cancer cell adhesion, invasion, and proliferation.

Harvesting Peritoneal Fat Depots: A Technique to Study Ovarian Cancer Colonization of Peritoneal Adipose Tissues in Mouse Models

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2023

In this video, we use a euthanized female mouse model bearing ovarian cancer. We then describe a protocol to identify and extract fat depots or adipose tissues associated with the five primary peritoneal organs to study metastatic cancer cell colonization.

Portal Vein Injection: A Method to Study Cancer Metastasis to the Liver

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2023

This video describes a surgical procedure to deliver mammary tumor cells to the murine liver via portal vein injection. This model permits the investigation of late stages of liver metastasis.

An Ex Vivo Model of Ovarian Cancer Peritoneal Metastasis Using Human Omentum

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2024

This protocol describes the establishment of a three-dimensional (3D) ex vivo model of cancer cell-omentum interaction. The model provides a platform for elucidating pro-tumor mechanisms within the adipose niche and for testing novel therapies.

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