Zebrafish Larvae Injection
Zebrafish Larvae Injection - Related Videos
02:28
Inducing Neuroinflammation in Zebrafish Larvae with a Lipopolysaccharide Injection
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2025
The video demonstrates the microinjection of lipopolysaccharides (LPS) into the brains of zebrafish larvae to induce neuroinflammation. The larvae are positioned on an agarose-lined dish, the needle is inserted into the brain ventricles, and the LPS is injected. The injected LPS triggers an immune response that leads to neuroinflammation.
10:53
Microgavage of Zebrafish Larvae
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Cited by 91 •
2013
We present a novel method for microgavage of larval zebrafish utilizing standard embryo microinjection and stereomicroscopy equipment. We demonstrate that microgavage is a safe and efficient technique useful for delivering controlled amounts of diverse materials specifically into the larval zebrafish intestinal lumen.
06:14
Automating Tumor Implantation in Zebrafish Larvae for Cancer Research and Medicine
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2025
Here, we present a detailed protocol for automated tumor xenograft implantation into zebrafish larvae using a state-of-the-art robotic injection system. Furthermore, we present injection videos and validation data demonstrating the system's successful performance.
02:24
A Technique to Quantify Fungal Infection in Zebrafish Larvae
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2025
This video demonstrates a method to quantify fungal infection in zebrafish larvae. Fungal spores are microinjected into the larval hindbrain ventricle, and the larvae are incubated to allow infection development. Infected larvae are harvested at defined intervals, and infection progress is assessed by enumerating colony-forming units.
10:45
Non-invasive Imaging of Disseminated Candidiasis in Zebrafish Larvae
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Cited by 33 •
2012
The rapid development, small size and transparency of zebrafish are tremendous advantages for the study of innate immune control of infection1-4. Here we demonstrate techniques for infecting zebrafish larvae using the fungal pathogen Candida albicans by microinjection, methodology recently used to implicate phagocyte NADPH oxidase activity in control of fungal dimorphism5.
FAQs
The injection site shapes the biological context in which introduced cancer cells are observed. Delivering material into the yolk sac, circulation, or surrounding tissues can support different questions about tumor growth, invasion, dissemination, or angiogenesis. Choosing the site therefore aligns the model with the specific cancer behavior being investigated and the anatomical setting most relevant to that question.
Fluorescent labels allow introduced cells to be tracked during larval development. This makes it possible to observe where cells remain, move, or spread and to relate their distribution to tumor growth, invasion, and dissemination. Combined with the larvae's transparency, fluorescence supports repeated imaging of cancer-cell behavior in a living organism rather than only in fixed samples.
Transparent zebrafish larvae make internal cancer-cell behavior accessible to imaging, while genetic tractability provides a system in which developmental and tumor-related questions can be examined. These features help researchers follow biological processes in vivo and connect visible tumor phenotypes with the broader experimental model. Together, they support rapid investigation of cancer mechanisms in developing organisms.
The fine glass needle provides the narrow delivery instrument, while the micromanipulator enables controlled positioning for precise placement in a selected anatomical site. Their combination is important because the study depends on introducing cells, drugs, or other materials into defined locations. Consistent placement helps researchers compare tumor-related outcomes across larvae and experimental conditions.
A typical workflow involves preparing the material to be delivered, positioning a fine glass needle with a micromanipulator, and introducing the material into a chosen site such as the yolk sac, circulation, or surrounding tissue. Researchers can then use imaging to follow fluorescently labeled cells and assess the resulting tumor-related behavior during development.
The approach can introduce cancer cells, drugs, or other experimental materials into developing zebrafish larvae. Cancer cells may be fluorescently labeled so their location and behavior can be monitored, whereas drug delivery supports evaluation of treatment effects. The selected material depends on whether the experiment focuses on tumor biology, therapeutic response, or another cancer-related process.
Injected larvae can support assessment of tumor growth, invasion, dissemination, angiogenesis, and treatment response. Because fluorescently labeled cells can be tracked in transparent animals, researchers can examine these outcomes in vivo during development. The resulting observations help connect the introduced material with visible changes in tumor behavior and provide a basis for comparing experimental conditions.
This approach is useful when researchers need an in vivo setting for rapid treatment assessment. Candidate drugs can be introduced alongside cancer cells or applied as an experimental treatment, after which researchers can monitor tumor-related behavior and treatment response. Its small size, accessible developmental stages, imaging compatibility, and suitability for drug screening support efficient comparison of therapeutic conditions.