Habituation Behavior

Habituation behavior is a basic form of non-associative learning in which an organism’s response to a repeated, harmless stimulus gradually decreases. Through repeated exposure, nervous systems adjust sensory processing and circuit activity, conserving attention for novel or biologically important signals; the response may return after rest or a meaningful change in stimulation. In developmental biology, habituation assays help researchers study the maturation of sensory and neural systems, as well as early learning and memory, across developing organisms.

Habituation Behavior - Related Videos

Education

JoVE Science Education - Basic Biology

Habituation and Positive Reinforcement for Rodent Handling

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2026

All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Habituation and positive reinforcement are refinement strategies that help reduce stress during routine handling and experimental procedures in rodents. These approaches improve animal welfare, encourage voluntary cooperation,...

Education

JoVE Science Education - Psychology
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Habituation: Studying Infants Before They Can Talk

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2023

Source: Laboratories of Nicholaus Noles, Judith Danovitch, and Cara Cashon—University of Louisville Infants are one of the purest sources of information about human thinking and learning, because they’ve had very few life experiences. Thus, researchers are interested in gathering data from infants, but as participants in experimental research, they are a challenging group to study. Unlike older children and adults, young infants are unable to reliably speak, understand speech, or even move and...

Research

JoVE Journal - Behavior

Testing for Odor Discrimination and Habituation in Mice

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Cited by 66 •

2015

This manuscript describes a protocol to examine the olfactory system of rodents. The olfactory habituation/dishabituation test will allow investigators to determine whether a mouse habituates to a repeatedly presented odor and whether the mouse demonstrates dishabituation when presented a novel odor.

Touch Habituation Assay: A Method to Study Non-Associative Learning in C. elegans

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2023

This video introduces the touch habituation assay, a C. elegans behavioral method of assessing mechanosensation and quantifying time to habituation.

Research

JoVE Journal - Neuroscience
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Habituation and Prepulse Inhibition of Acoustic Startle in Rodents

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Cited by 164 •

2011

Habituation and prepulse inhibition of startle are operational measures of sensory gating. Sensory gating is disrupted in schizophrenia, and some other mental disorders and neurodegenerative diseases. We here describe a standard protocol to assess short-term and long-term habituation as well as prepulse inhibition of acoustic startle responses in rats and mice.

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