Tissue Culture Reactor
Tissue Culture Reactor - Related Videos
08:45
Whole Organ Tissue Culture
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2023
Whole organs can be cultured ex vivo using specialized bioreactors with the goal of repairing or replacing entire organs. This method uses a donor organ that is stripped of all cells, leaving behind the three-dimensional structure, which is then repopulated with new cells. This video demonstrates the whole organ culture of lungs and shows how a dynamic culture that mimics the mechanical stimulation in the body is needed to induce native tissue properties.
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Operation of High-pressure Reactor Vessels
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2023
Robert M Rioux, Pennsylvania State University, University Park, PA The use of gases in a synthetic chemistry laboratory is essential for carrying out a variety of highly facile and atom economical transformations. Reactions such as hydrogenation, oxidation, and amination require the use of gases like hydrogen, oxygen, and ammonia. Due to the poor solubility of these gases in typical reactant solutions, high pressures are necessary to achieve a meaningful reaction rate. Not only are these gases...
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Catalytic Reactor: Hydrogenation of Ethylene
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2023
Source: Kerry M. Dooley and Michael G. Benton, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA The hydrogenation of ethylene (C2H4) to ethane (C2H6) has often been studied as a model reduction reaction in characterizing new metal catalysts.1-2 While supported nickel is not the most active metal catalyst for this reaction, it is active enough that reaction can take place at < 200°C. The reaction typically involves adsorbed, dissociated hydrogen (H2) reacting...
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Liquid Phase Reactor: Sucrose Inversion
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2023
Source: Kerry M. Dooley and Michael G. Benton, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA Both batch and continuous flow reactors are used in catalytic reactions. Packed beds, which use solid catalysts and a continuous flow, are the most common configuration. In the absence of an extensive recycle stream, such packed bed reactors are typically modeled as "plug flow". The other most common continuous reactor is a stirred tank, which is assumed to be perfectly...
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Developing a Bacterial Monolayer Biofilm Using an Electrochemical Reactor
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2026
Source: Tokunou, Y. et. al., Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1. J. Vis. Exp. (2018)This video demonstrates the assembly and operation of an electrochemical reactor model designed to simulate biofilm formation on electrode surfaces. Controlled electrochemical stimulation promotes microbial adhesion, leading to the gradual development of a bacterial monolayer biofilm.
FAQs
Control depends on coordinating several environmental variables rather than adjusting a single condition. Nutrient delivery, oxygenation, temperature, pH, and fluid flow shape the conditions surrounding cells or tissues, while mechanical stimulation can influence organization and development. Engineering these inputs together helps maintain cultures and creates a controlled setting for examining how cells respond to their environment.
Shear forces are a critical design consideration because moving fluid can affect cell behavior. A reactor must manage flow strongly enough to support delivery and oxygenation without imposing unintended mechanical effects on the culture. The appropriate balance depends on the biological construct and the experiment, making fluid movement and mechanical stimulation important engineering variables rather than simple operating details.
Compared with static culture, a tissue culture reactor can provide a more uniform environment and offers greater potential for scale. This difference matters when researchers need controlled exposure to nutrients, oxygen, flow, or mechanical cues across a culture. The reactor supports experiments that examine tissue development or cell-material interactions under conditions less dependent on local variation.
An engineering workflow begins by establishing the biological culture and selecting reactor conditions needed for its maintenance, growth, or organization. Researchers then regulate nutrient delivery, oxygenation, temperature, pH, fluid flow, and, when appropriate, mechanical stimulation. Throughout the experiment, they must also consider shear forces because these conditions can alter cell behavior and tissue organization.
Researchers apply these systems in tissue engineering and regenerative medicine to develop functional biological constructs. They also use them for disease modeling, where controlled culture conditions can support investigation of tissue behavior, and for studies of cell-material interactions. These applications benefit from exposing living cells and tissues to engineered environmental and mechanical conditions rather than relying only on static culture.
Controlled culture can support tissue organization and the development of functional biological constructs. In engineering studies, researchers can relate environmental inputs, fluid conditions, and mechanical stimulation to tissue development. This makes the reactor useful for evaluating how design choices influence a construct’s biological behavior and for studying the conditions that support organized, functional tissue growth.