Mbt/cbt Proxy
Mbt/cbt Proxy - Related Videos
11:54
Real-Time Proxy-Control of Re-Parameterized Peripheral Signals using a Close-Loop Interface
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Cited by 1 •
2021
We present protocols and methods of analyses to build co-adaptive interfaces that stream, parameterize, analyze, and modify human body and heart signals in close-loop. This setup interfaces signals derived from the peripheral and central nervous systems of the person with external sensory inputs to help track biophysical change.
09:21
Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
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Cited by 14 •
2018
The protocol described here is based on the genome-wide quantification of newly synthesized mRNA purified from yeast cells labeled with 4-thiouracil. This method allows to measure mRNA synthesis uncoupled from mRNA decay and, thus, provides an accurate measurement of RNA polymerase II transcription.
08:28
An Overview of bGDGT Biomarker Analysis for Paleoclimatology
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2023
Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst Throughout this series of videos, natural samples were extracted and purified in search of organic compounds, called biomarkers, that can relate information on climates and environments of the past. One of the samples analyzed was sediment. Sediments accumulate over geologic time in basins, depressions in the Earth into which sediment flows through the action of fluid (water or air), movement, and gravity. Two main types...
07:52
Removal of Branched and Cyclic Compounds by Urea Adduction for Uk'37 Paleothermometry
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2023
Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst As mentioned in previous videos, the product of an organic solvent extraction, a total lipid extract (TLE), is often a complex mixture of hundreds, if not thousands, of different compounds. The researcher is often only interested in a handful of compounds. In the case of our two organic paleothermometers (Uk'37 and MBT/CBT), the interest is in only 6 compounds (2 alkenones and 4 isoprenoidal glycerol dialkyl glycerol...
04:02
Drosophila In Vivo Calcium Imaging: A Method for Functional Imaging of Neuronal Activity
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2023
This video describes in vivo calcium imaging in Drosophila neurons using GCaMP. The featured protocol clip shows how to record changes in GCaMP fluorescence from mushroom body neurons during an olfactory conditioning experiment.
FAQs
The two structural signals provide different environmental information. Relative methylation of branched tetraethers varies primarily with temperature, whereas the number of cyclopentane rings, summarized through cyclization, tracks pH in many calibrated settings. Evaluating both patterns helps researchers distinguish temperature-related changes from soil or depositional acidity and interpret environmental shifts more carefully.
Regional calibrations translate measured brGDGT distributions into estimates of temperature and pH for a particular environmental setting. This step matters because the proxy is interpreted in soils, lake sediments, and marine deposits, where depositional contexts differ. Applying the calibration suited to the archive helps researchers estimate conditions without treating all settings identically.
Archive choice establishes the environmental context for the measurement. Soil samples relate the signal to soil conditions, whereas lake sediments and marine deposits preserve information about depositional environments. Researchers therefore interpret the temperature and pH estimates alongside whether compounds came from soil, a lake, or a marine setting.
Researchers quantify the relative abundances of brGDGT structures in a sample using chromatographic and mass-spectrometric methods. They then evaluate methylation and cyclization patterns and apply a regional calibration to estimate temperature and soil or depositional pH. The resulting values support reconstructions of past environmental conditions over time.
These records can be used to examine past climate change, hydrology, weathering, and ecosystem responses. Because the proxy provides estimates tied to temperature and soil or depositional pH, it links molecular measurements with broader environmental processes. In Environmental Sciences, that connection supports interpretation of how climate and landscape conditions changed across geological timescales.
These records provide a way to compare reconstructed temperature and soil or depositional pH across long intervals of Earth history. That perspective helps distinguish individual environmental conditions from broader patterns in climate, hydrology, weathering, and ecosystem response. It also makes the proxy relevant to questions that cannot be addressed by short-term environmental observations alone.