Intrapulmonary Delivery
Intrapulmonary Delivery - Related Videos
07:56
Isolation of Intrapulmonary Artery and Smooth Muscle Cells to Investigate Vascular Responses
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2022
Vascular responses of arterial pulmonary circulation can be explored using intrapulmonary artery (IPA) and vascular smooth muscle cells (VSMCs). The present study describes the isolation of IPA in detail and the protocols used for investigating vasorelaxation in response to physiological stimuli.
06:15
Murine Intrapulmonary Tracheal Transplantation: A Model for Investigating Obliterative Airway Disease After Lung Transplantation
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2023
The murine intrapulmonary tracheal transplantation (IPTT) model is valuable for studying obliterative airway disease (OAD) after lung transplantation. It offers insights into lung-specific immunological and angiogenic behavior in airway obliteration after allotransplantation with high reproducibility. Here, we describe the IPTT procedure and its expected results.
07:09
Assessment of Pulmonary Capillary Blood Volume, Membrane Diffusing Capacity, and Intrapulmonary Arteriovenous Anastomoses During Exercise
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Cited by 11 •
2017
To assess the pulmonary diffusion and vasculature responses to exercise, we describe the multiple-inspired oxygen diffusion capacity technique to determine capillary blood volume and membrane diffusing capacity, as well as agitated saline contrast echocardiography to assess the recruitment of intrapulmonary arteriovenous anastomoses.
06:30
Intraductal Delivery to the Rabbit Mammary Gland
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Cited by 9 •
2017
Here, we describe a technique for the localized delivery of reagents to the rabbit mammary gland via an intraductal injection. In addition, we describe a protocol for visualization and the confirmation of delivery by high-resolution ultrasound imaging of contrast agents.
08:02
Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
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Cited by 40 •
2013
Rapid mechanical deformation of cells has emerged as a promising, vector-free method for intracellular delivery of macromolecules and nanomaterials. This protocol provides detailed steps on how to use the system for a broad range of applications.
FAQs
Particle characteristics and airway anatomy help determine where an inhaled formulation deposits within the respiratory tract. Depending on these factors, material may reach the bronchi or alveoli rather than remaining distributed uniformly throughout the lungs. This location-specific deposition matters because it can influence which respiratory tissues receive treatment and how researchers interpret local therapeutic or immune effects.
The deposition site affects whether an agent acts near the respiratory interface or produces effects beyond the lungs. Delivery to different pulmonary regions can therefore change treatment efficacy, safety, and the pattern of immune interaction. In infection and immunology studies, identifying the affected region helps connect delivery location with antimicrobial activity or the strength and distribution of immune protection.
Breathing patterns influence how inhaled formulations move through the airways and where they ultimately deposit. Because airway distribution depends partly on the way the formulation is inhaled, differences in breathing can change exposure of bronchial or alveolar regions. Accounting for this variable is important when comparing treatments, evaluating pulmonary responses, or interpreting differences in delivery performance.
A basic workflow identifies the therapeutic agent or vaccine, administers it through an inhaled formulation, and then examines its distribution within the lungs. Researchers can relate deposition in the bronchi or alveoli to local or systemic effects. In immunology and infection studies, this approach supports assessment of treatment efficacy, safety, mucosal responses, or immune protection.
Researchers may choose this approach when they need to investigate respiratory pathogens or evaluate therapy at the respiratory interface. It can support studies of antimicrobial treatment by placing the intervention within pulmonary regions affected by infection. The resulting observations help determine how delivery location relates to therapeutic efficacy, safety, and responses associated with respiratory disease.
In pulmonary vaccination research, direct delivery to the lungs allows investigators to examine immune protection in the respiratory system and study mucosal immune responses. Researchers can compare how vaccine deposition in regions such as the bronchi or alveoli relates to protection and local effects. These findings help evaluate whether delivery location contributes to the observed immune outcome.