Patient Blood Samples
Patient Blood Samples - Related Videos
07:32
Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples
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Cited by 36 •
2012
Circulating tumor cells are isolated from the blood of cancer patients without inflicting cellular damage. Isolation of tumor cells is accomplished using a bimolecular surface of E-selectin in addition to antibodies against epithelial markers. A nanotube coating specifically promotes cancer cell adhesion resulting in high capture purities.
08:25
Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients
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Cited by 6 •
2020
In this paper we present a detailed protocol for non-invasive liquid biopsy technique, including blood collection, plasma and buffy coat separation, cfDNA and germline DNA extraction, quantification of cfDNA or germline DNA, and cfDNA fragment enrichment analysis.
07:11
Sampling Blood from the Lateral Tail Vein of the Rat
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Cited by 82 •
2015
Blood samples are useful for assessing biomarkers of physiological states or disease in vivo. Here we describe the methodology to sample blood from the lateral tail vein in the rat. This method provides rapid samples with minimal pain and invasiveness.
13:50
Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients
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Cited by 7 •
2011
Our experiment will show how to perform a sequencing analysis of bacterial species translocating in peripheral blood of HIV positive patients.
03:50
CTC Isolation from a Whole Blood Sample: A Method to Obtain CTCs from Murine Colorectal Cancer Model
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2023
This video describes a detailed protocol for isolating circulating tumor cells from the whole blood sample of a euthanized colorectal tumor mouse model. These isolated CTCs can be cultured and used for intended downstream analysis.
FAQs
Sample integrity affects whether physiological, cellular, and molecular measurements accurately represent the individual at collection. Delays or unsuitable handling can compromise the usefulness of separated plasma, serum, or immune-cell preparations, making assay results less reliable. Consistent collection, processing, storage, and documentation therefore support valid comparisons across participants and improve confidence in clinical and translational findings.
Different blood fractions provide access to different kinds of evidence. Plasma or serum can support analysis of soluble features such as antibodies and cytokines, whereas immune-cell preparations allow investigators to examine blood-cell populations. Selecting the relevant component helps align the specimen with the intended assay, whether the goal is immune profiling, pathogen assessment, or response monitoring.
Documentation connects each specimen to its collection and laboratory history. Recording relevant handling information helps investigators interpret differences in assay results, identify potential sources of variability, and maintain consistency across a study. This is especially important when samples are compared between participants or across stages of diagnosis, treatment monitoring, vaccination research, or therapeutic evaluation.
A general workflow includes collecting the specimen, separating it into appropriate components, preserving those components under suitable storage conditions, and applying assays matched to the scientific question. Investigators then interpret measurements such as antibodies, cytokines, immune-cell populations, or evidence of infectious agents. Careful documentation accompanies each stage to protect traceability and sample quality.
These specimens are useful when a study needs clinically relevant evidence of immune activity or infection. Applications include supporting diagnosis, detecting infectious agents, characterizing immune profiles, monitoring treatment, and assessing responses to vaccines or therapeutics. Because several measurement types can be obtained from related blood components, one study can address complementary cellular, molecular, and physiological questions.
Researchers can compare immune or infection-related measurements in samples collected during different stages of a clinical study. Antibodies, cytokines, immune-cell populations, or evidence of infectious agents may provide distinct indicators of response. The resulting profiles can help evaluate whether an intervention is associated with measurable changes and can connect laboratory findings with broader clinical research objectives.