Rapid Molecular Testing
Rapid Molecular Testing - Related Videos
13:32
Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling
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Cited by 18 •
2018
The main purpose of this study was to adapt the needle immersed vitrification (NIV) procedure to cryopreserve whole zebrafish testes. Additionally, the repeatability of the method in five different zebrafish strains was tested.
05:38
Rapid Molecular Detection and Differentiation of Influenza Viruses A and B
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Cited by 4 •
2017
We describe a rapid, molecular-based Influenza A and B assay. The Influenza assay detects each target within 15 min by employing isothermal amplification with influenza-specific primers followed by target detection with molecular beacon probes. The Influenza A and B assay is user-friendly and required minimal hands-on time to perform.
09:55
Molecular Cloning
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2023
Molecular cloning is a set of methods, which are used to insert recombinant DNA into a vector - a carrier of DNA molecules that will replicate recombinant DNA fragments in host organisms. The DNA fragment, which may be a gene, can be isolated from a prokaryotic or eukaryotic specimen. Following isolation of the fragment of interest, or insert, both the vector and insert must be cut with restriction enzymes and purified. The purified pieces are joined together though a technique called...
06:48
Rapid Formation and Testing of Self-expanding NiTi Frames with a Small Form Factor Suitable for Minimally Invasive Implants
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Cited by 1 •
2025
This work illustrates a low-cost fabrication technique for shape-setting nitinol wires/frames with a small form factor using sacrificial fixtures. The technique is demonstrated for the fabrication of self-expanding frames designed for minimally invasive implants with complex shapes.
09:10
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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Cited by 17 •
2010
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
FAQs
Target selection determines what an assay can reveal and how specifically it identifies an infection. Sequence-directed components focus amplification on pathogen-specific genetic material, while fluorescent probes or related detection systems convert the amplified target into a measurable signal. Selecting an appropriate target helps distinguish the suspected organism from unrelated material and supports interpretation alongside specimen quality and assay performance.
PCR and isothermal amplification provide alternative ways to increase the amount of pathogen-specific DNA or RNA so it can be detected. After amplification, the assay generates a signal through fluorescence or a related detection system. These components connect the molecular target to a readable result, allowing laboratories and clinical teams to obtain actionable information without relying solely on organism growth.
A molecular result depends on whether the patient specimen adequately represents the suspected infection and whether the assay performs reliably for its selected target. Poor specimen quality can limit detection, while inappropriate target selection or weaker assay performance can affect interpretation. Consequently, rapid results should be considered with the specimen context and the clinical or research question they are intended to address.
The workflow begins with collecting a patient specimen and extracting its DNA or RNA. The selected genetic sequence is then amplified by PCR or isothermal amplification, followed by signal generation through fluorescent probes or a related detection system. The resulting finding can support rapid identification of an infection, provided that specimen quality, target selection, and assay performance are suitable.
It is particularly useful when timely identification can influence treatment or infection-control decisions, when the suspected organism is difficult to culture, or when researchers need to distinguish active infection from an immune response. By detecting pathogen-specific genetic material directly, the approach can provide information that complements immunological findings and supports more immediate decisions than culture-dependent investigations alone.
Rapid molecular testing can identify pathogen-specific genetic material across specimens, helping investigators recognize infections during transmission monitoring and outbreak surveillance. The speed of results supports timely assessment of emerging infection patterns and can inform infection-control measures. Interpretation still depends on the selected target, specimen quality, and assay performance, so molecular findings must be evaluated within the surveillance context.