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Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics
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Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics

Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics

DOI:
10.3791/1086-v

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13:51 min

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February 18, 2009

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, , , ,
1Department of Marine Sciences,University of Georgia (UGA)

Chapters

  • 00:00Title
  • 01:06Introduction
  • 01:51Preparation and Environmental RNA Collection
  • 03:32Total RNA Extraction
  • 06:03First rRNA Removal
  • 07:29Second rRNA Removal
  • 10:38mRNA Amplification
  • 12:01cDNA Synthesis
  • 12:51Representative Results/ Outcome
  • 13:16Conclusion

Summary

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We present a method for generating cDNA from environmental mRNA. In general, total RNA is first collected from the environment, rRNA is selectively removed, mRNA is selectively amplified, and cDNA synthesized from the enriched mRNA pool is sequenced. Recovered sequences can be annotated using standard bioinformatics techniques to identify the expressed genes.

Tags

Gene ExpressionMarine Microbial CommunitiesEnvironmental TranscriptomicsMetatranscriptomicsMRNA SequencingProkaryotic MRNAPoly(A) TailsEukaryotic MessagesClone LibrariesRandom PrimersCDNA SynthesisLinear AmplificationIn Vitro Transcription

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