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Chapters
- 00:05Title
- 00:54Introduction
- 01:27Transfection of Neurons Using Lipofectamine 2000
- 04:09Live Imaging of GFP-Tagged Dense-Core Vesicles in Cultured Hippocampal Neurons
- 08:25Representative Results
- 09:12Conclusion
Live cell imaging is of particular utility when studying the dynamics of organelle trafficking. Here we describe a protocol for live imaging of dense-core vesicles in cultured neurons using wide-field fluorescence microscopy. This protocol is flexible and can be adapted to image other organelles such as mitochondria, endosomes, and peroxisomes.
Tags
Live ImagingDense-core VesiclesPrimary Cultured Hippocampal NeuronsFluorescent ProbesGreen Fluorescent Protein (GFP)Cell BiologyDynamic Cellular ProcessesIntracellular CompartmentsCellular StructuresCytoskeletonOrganelle TransportMembrane DynamicsTechnical ChallengesPost-mitotic NeuronsPhototoxicityPhotobleachingCell HealthLipid-based Transfection MethodTransfection RatePolarized NeuronsAxonsDendritesDirectionality Of Transport
