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Biology

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Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons
 

Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons

Article DOI: 10.3791/1144
May 29th, 2009

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Summary May 29th, 2009

Live cell imaging is of particular utility when studying the dynamics of organelle trafficking. Here we describe a protocol for live imaging of dense-core vesicles in cultured neurons using wide-field fluorescence microscopy. This protocol is flexible and can be adapted to image other organelles such as mitochondria, endosomes, and peroxisomes.

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