Journal
/
/
Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy
DOI:

07:11 min

April 01, 2009

,

Chapters

  • 00:00Title
  • 00:37Introduction
  • 01:09Preparing for Injection
  • 02:14Injecting mRNA
  • 04:12Mounting and Imaging Embryos
  • 06:14Representative Results: Confocal Imaging
  • 06:35Conclusion

Summary

Automatic Translation

In this video, we demonstrate a method by which to analyze the developing vertebrate brain in live zebrafish embryos at single cell resolution by confocal microscopy. This includes the method by which we inject the single-cell zebrafish embryo and subsequently mount and image the developing brain.

Related Videos

Read Article