6.10
Mechanical Protein Function
Proteins perform a variety of mechanical functions including those that produce cell movement and muscle contraction, as well as the transport of molecules between different locations inside and outside of a cell.
Mechanical protein functions are powered by the conversion of chemical energy to mechanical work through conformational changes in protein structure.
Within a protein domain, simple chemical changes, like the hydrolysis of bound molecules such as ATP or GTP, can result in conformational changes that create much larger movements in the protein.
ATP hydrolysis powers the motor protein myosin, which acts as a lever, pulling actin filaments and causing muscles to contract. Helicases that unwind DNA during replication and transcription also use ATP to break hydrogen bonds and move along the DNA strand.
In the ribosome, many proteins work together as a machine to synthesize new proteins. GTP hydrolysis allows the elongation factor, EF-Tu to transfer a tRNA molecule to the ribosome so it can add an amino acid to the growing protein strand.
tRNA is associated with EF-Tu when GTP is bound. When GTP is hydrolyzed to GDP, the release of the phosphate group results in a small conformation change in and near the nucleotide-binding site.
This small shift causes an α helix located at the interface of the GTPase domain and the other two domains to change positions. The movement of this helix causes two domains to swing open to release the bound tRNA.
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.
Functions such as cell movement and muscle contraction require the conversion of chemical energy to a mechanical, usually through conformational changes. For example, hydrolysis of nucleoside triphosphates, such as ATP and GTP, can result in a small conformational change that gets amplified to major structural changes. For example, EF-Tu is a protein with three distinct domains that transfers a tRNA molecule to the ribosome. One of the domains binds GTP, and the hydrolysis of GTP to GDP results in a conformational change in the nucleotide-binding site due to the released inorganic phosphate. This triggers the movement of an alpha-helix that is located at the interface of the GTP domain and the other two domains changing the relative position of the domains to each other. This allows the protein to release the tRNA that is held at the interface by the three domains, thereby allowing it to move into the ribosome.
Some proteins, such as actin, provide many types of mechanical functions. For example, actin acts as a track for the mechanical protein myosin to walk along. Depending on the type, myosin can perform various functions, such as either pulling on the actin filaments or transporting an attached organelle along the filament. As part of the cytoskeleton, actin filaments act as a mechanical support for the cell structure. During cell movement, these filaments exert pressure on the cell membrane causing the cell to form filopodia and lamellipodia, extensions of the cell membrane that allow the cell to migrate to a new location. Scientists have developed techniques, such as optical tweezers, that can measure the force that actin produces when deforming the membrane.
Proteins perform a variety of mechanical functions including those that produce cell movement and muscle contraction, as well as the transport of molecules between different locations inside and outside of a cell.
Mechanical protein functions are powered by the conversion of chemical energy to mechanical work through conformational changes in protein structure.
Within a protein domain, simple chemical changes, like the hydrolysis of bound molecules such as ATP or GTP, can result in conformational changes that create much larger movements in the protein.
ATP hydrolysis powers the motor protein myosin, which acts as a lever, pulling actin filaments and causing muscles to contract. Helicases that unwind DNA during replication and transcription also use ATP to break hydrogen bonds and move along the DNA strand.
In the ribosome, many proteins work together as a machine to synthesize new proteins. GTP hydrolysis allows the elongation factor, EF-Tu to transfer a tRNA molecule to the ribosome so it can add an amino acid to the growing protein strand.
tRNA is associated with EF-Tu when GTP is bound. When GTP is hydrolyzed to GDP, the release of the phosphate group results in a small conformation change in and near the nucleotide-binding site.
This small shift causes an α helix located at the interface of the GTPase domain and the other two domains to change positions. The movement of this helix causes two domains to swing open to release the bound tRNA.
- Mechanical Support - In biology, it allows certain structures within cells to maintain their shape.
- Protein Function - Proteins carry out many functions within a cell, from mechanical support to movement.
- Cell Movement - A process guided by proteins, helping cells to move or change shape.
- Functional Proteins - These are proteins that carry out specific jobs within cells, such as cell movement or providing mechanical support.
- Protein Synthesis Enzymes - These help the cells to produce the functional proteins necessary for various cell functions.
- Define Mechanical Support - This is to understand its role in maintaining cell integrity and structure (e.g., mechanical support).
- Contrast Function of Proteins in Movement vs Structural Support - Analyze how proteins can both aid cell movement and provide structural integrity (e.g., cell movement, mechanical support).
- Explore Protein Function Examples - Understand specific examples of proteins in action (e.g., functional proteins, protein synthesis enzymes).
- Explain Protein Synthesis Process - Understand the role and process of protein synthesis in the producing functional proteins.
- Apply Protein Knowledge in Biological Context - Get an understanding of how protein knowledge is applied in biological context.
- What is the meaning of mechanical support in biology?
- How does protein function in cell movement and providing mechanical support?
- What are functional proteins and what are their examples?
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