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Geração de humanos CD40-activated células B
JoVE Journal
Biology
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JoVE Journal Biology
Generation of Human CD40-activated B cells
DOI:

13:27 min

October 16, 2009

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Chapters

  • 00:00Title
  • 00:14Introduction
  • 01:46Preparation of Feeder Cells
  • 02:27Preparation of PBMC for CD40- Stimulation
  • 04:38Preparation of Feeder Cells
  • 08:33CD40- Stimulation
  • 09:50Subcultivation
  • 12:20Analysis of CD40- Activated B Cells
  • 12:55Conclusion

Summary

Automatic Translation

Neste vídeo, apresentamos a<em> Ex vivo</em> Geração e expansão de células CD40-activated B (CD40-B) a partir de células mononucleares do sangue periférico (PBMC) de estimulação com CD40 ligante e interleucina-4.

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