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Generating Chimeric Zebrafish Embryos by Transplantation
JoVE Journal
Biology
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JoVE Journal Biology
Generating Chimeric Zebrafish Embryos by Transplantation
DOI:

21:01 min

July 17, 2009

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Chapters

  • 00:05Title
  • 00:13Introduction
  • 01:12Injecting Zebrafish Embryos at the 1-cell Stage
  • 06:15Preparing the Transplantation Rig and Needle
  • 08:31Transplanting Cells between Bastula Stage Embryos
  • 13:00Transplanting Primordial Germ Cells at the Blastula Stage
  • 15:02Transplating Cells Between Gastrula Stage Embryos
  • 18:46Representative Results/ Outcome
  • 20:03Conclusion

Summary

Automatic Translation

A step-by-step guide to generating targeted chimeric zebrafish embryos by transplantation at the blastula or gastrula stage.

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