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JoVE Journal
Biology

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Lentivirus Production
 

Lentivirus Production

Article DOI: 10.3791/1499
October 2nd, 2009

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Summary October 2nd, 2009

To make lentiviruses, DNA vectors are transfected into human 293 cells. After harvest and concentrating the supernatant, virus titer is determined by fluorescence expression with a flow cytometer.

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