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Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
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Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures

Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures

DOI:
10.3791/1523-v

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08:32 min

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October 30, 2009

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,
1Stem Cell Research Unit,Medical University of Graz, Austria

Chapters

  • 00:00Title
  • 00:55Introduction
  • 01:32Preparation of Human Platelet Lysate
  • 06:36Characteristics of the Resulting Lysate
  • 08:02Conclusion

Summary

Automatic Translation

Automatic Translation

Human platelet lysate is a rich source of growth factors and a potent supplement in cell culture. This protocol presents the process of preparing a large pool of human platelet lysate by starting from platelet rich plasma, performing several freeze-thaw cycles and depleting the platelet fragments.

Tags

Pooled Human Platelet LysatePHPLAnimal Serum-freeStem Cell CulturesPlatelet Derived Growth FactorsCell ProliferationMesenchymal Stromal CellsFibroblastsEndothelial Colony-forming CellsThrombin ActivationFreeze/thaw CyclesPlatelet ReleasateTrophic EffectRegenerative TherapyGrowth Factor ConcentrationsStandardized ProtocolsPlatelet PreparationHuman Platelet LysatePlatelet Rich PlasmaFreeze/thaw CyclesCentrifugationAggregate FormationPotential AntigensCulture ProtocolsCell Therapeutics

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