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Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
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Biology
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Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

DOI:
10.3791/1529-v

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15:57 min

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October 09, 2009

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, ,
1Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School

Chapters

  • 00:00Title
  • 00:42Introduction
  • 01:11Preparing the DNA Replication Template
  • 02:44Preparing Functionalized Coverslips
  • 05:46Assembling the Flow Chamber
  • 10:03Single-Molecule Replication Experiment
  • 13:18Single Molecule DNA Replication Data Analysis and Representative Results
  • 15:00Conclusion

Summary

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Automatic Translation

This protocol demonstrates a simple single-molecule fluorescence microscopy technique for visualizing DNA replication by individual replisomes in real time.

Tags

Single-molecule DNA ReplicationFluorescence MicroscopyReal-time MethodCircular DNA TemplateReplication ProteinsNucleotidesGrowing DNA EndReplication RateCompleted DNA ProductsProcessivity Of ReplicationIntercalating DyeFluorescence Microscope

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