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Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
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Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Article DOI: 10.3791/1529 • 15:58 min • October 9th, 2009
October 9th, 2009 •
1, 1, 1
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School

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Summary
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This protocol demonstrates a simple single-molecule fluorescence microscopy technique for visualizing DNA replication by individual replisomes in real time.

Tags

Single-molecule DNA Replication Fluorescence Microscopy Real-time Method Circular DNA Template Replication Proteins Nucleotides Growing DNA End Replication Rate Completed DNA Products Processivity Of Replication Intercalating Dye Fluorescence Microscope
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