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Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
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Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell

Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell

DOI:
10.3791/1530-v

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11:36 min

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November 05, 2009

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, , ,
1National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health,Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science

Chapters

  • 00:00Introduciton
  • 00:21Materials
  • 02:21Sample Cell Assembly and Torque
  • 07:07Loading and Sealing the Sample Cell
  • 09:23Counterbalance and Sample Cell Alignment

Summary

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The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.

Tags

Analytical UltracentrifugeAUCSedimentation VelocitySedimentation EquilibriumProtein AnalysisSample Cell AssemblyWindow AssembliesTorque PressureReference BufferSample LoadingAlignmentTitanium RotorOptical Detection SystemsHigh VacuumSample Cell ComponentsHandling Precautions

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