Journal
/
Biology
/
The use of SC1 (Pluripotin) to Support mESC Self-renewal in the Absence of LIF
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
The use of SC1 (Pluripotin) to Support mESC Self-renewal in the Absence of LIF

The use of SC1 (Pluripotin) to Support mESC Self-renewal in the Absence of LIF

DOI:
10.3791/1550-v

•

05:58 min

•

November 18, 2009

•

, , , , , , ,
1Research and Development,Stemgent, 2Product Marketing,Stemgent

Chapters

  • 00:00Title
  • 00:08Introduction
  • 00:51Maintenance of Self-Renewal of Mouse Embryonic Stem Cells
  • 03:11Immunocytochemical Examination of Pluripotency Markers
  • 04:54Representative Images of Mouse Stem Cells Grown in the Presence of SC1
  • 05:28Conclusion

Summary

Automatic Translation

Automatic Translation

SC1 functions through dual inhibition of Ras- GAP and ERK1. We tested the function of SC1 in supporting mouse ES cell self-renewal in the absence of LIF and showed that SC1 is able to maintain self-renewal of mouse ES cell cultures.

Tags

SC1PluripotinMESC Self-renewalLIFSC1 Mechanism Of ActionRas-GAPERK1 InhibitionEconomical Alternative To LIFMolecular Mechanism Of Self-renewalCulturing Mouse ES Cells With SC1Morphology Of Mouse ES CellsPluripotency MarkersOct4Sox2NanogSSEA1Toxicity On Mouse ES Cells

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/1550?article_type=v

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
check_url/1550?article_type=v

The use of Biofeedback in Clinical Virtual Reality: The INTREPID Project
check_url/1550?article_type=v

Quantification of γH2AX Foci in Response to Ionising Radiation
check_url/1550?article_type=v

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
check_url/1550?article_type=v

The Use of Chemostats in Microbial Systems Biology
check_url/1550?article_type=v

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
check_url/1550?article_type=v

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
check_url/1550?article_type=v

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney
check_url/1550?article_type=v

Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact
check_url/1550?article_type=v

The Use of Ex Vivo Whole-organ Imaging and Quantitative Tissue Histology to Determine the Bio-distribution of Fluorescently Labeled Molecules

Read Article