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Chapters
- 00:00Title
- 00:58Introduction
- 01:31Selecting and Washing Larvae
- 02:27Dissecting the Larvae and Removing Non-Specific Cells
- 04:47Dissociating the Tissue into a Single Cell Suspension
- 08:07Magnetic Bead Cell Sorting
- 10:05Representative Results of DA Neurons Isolation and RNA Extraction
- 11:15Conclusion
Neste vídeo-artigo apresentamos um método para o isolamento e purificação de<em> Drosophila</em> Neurônios periféricos usando um jejum magnética estratégia de triagem talão assistida celular. RNA obtido a partir de células isoladas podem ser facilmente usados para aplicações a jusante, incluindo análises microarray.
Tags
IsolationPurificationDrosophila Peripheral NeuronsMagnetic Bead SortingPNS NeuronsDendritic Arborization (da) NeuronsNeuronal DevelopmentDendrite MorphogenesisFunctional And Molecular LevelsPure PopulationLow OccurrenceTough Chitinous Larval CuticleSpecific Cell EnrichmentAntibody-coated Magnetic BeadsAge-matched Third Instar LarvaeMouse CD8 Tagged GFP Fusion Protein (UAS-mCD8-GFP)Pickpocket (ppk)-GAL4 DriverPan-da Neuron-specific GAL421-7 DriverLarval CuticleCell Types
