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JoVE Journal
Biology

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Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons
 

Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons

Article DOI: 10.3791/1614
December 10th, 2009

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Summary December 10th, 2009

Direct intranuclear injection of cDNA is an effective transfection technique for post-mitotic cells. This method provides high levels of heterologous protein expression from single or multiple cDNA constructs and enables protein function to be studied in a physiologically relevant environment with a variety of single cell assays.

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