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Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching
 

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

Article DOI: 10.3791/1636
February 26th, 2010

Chapters

Summary February 26th, 2010

In this article we will describe the procedure for measuring diffusion coefficients using multi-photon fluorescence recovery after photobleaching. We will begin by aligning the laser along the optical path to the sample and determining the proper experimental parameters, then continue generating and finally fitting fluorescence recovery curves.

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