Journal
/
Biology
/
A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device

A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device

DOI:
10.3791/1649-v

•

07:55 min

•

January 26, 2010

•

, , , , ,
1Department of Surgery,University of Illinois, Chicago, 2Department of Bioengineering,University of Illinois, Chicago

Chapters

  • 00:00Title
  • 00:34Introduction
  • 01:10Microfabrication of 3-layer Microfluidic Perifusion Device
  • 02:18Simultaneous Perifusion and Imaging of Mouse Islets
  • 04:45Islet Perifusion
  • 06:55Simultaneous Monitoring of Multiple Parameters in Stimulated Islets
  • 07:23Conclusion

Summary

Automatic Translation

Automatic Translation

A microfluidic islet perifusion device was developed for the assessment of dynamic insulin secretion of multiple islets and simultaneous fluorescence imaging of calcium influx and mitochondrial potential changes.

Tags

Microfluidic Islet Perifusion DeviceDynamic Insulin SecretionFluorescence ImagingCalcium InfluxMitochondrial Potential ChangesMicroscale WellsPerifusion ChamberInlet-mixing ChannelGlucose GradientsLinear ShapeBell ShapeSquare Shape

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/1649?article_type=v

Murine Pancreatic Islet Isolation
check_url/1649?article_type=v

Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons
check_url/1649?article_type=v

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior
check_url/1649?article_type=v

A Gradient-generating Microfluidic Device for Cell Biology
check_url/1649?article_type=v

Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device
check_url/1649?article_type=v

Cell Capture Using a Microfluidic Device
check_url/1649?article_type=v

Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device
check_url/1649?article_type=v

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform
check_url/1649?article_type=v

The Microfluidic Probe: Operation and Use for Localized Surface Processing
check_url/1649?article_type=v

Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions

Read Article