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Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques
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Immunology and Infection
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JoVE Journal Immunology and Infection
Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques

Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques

DOI:
10.3791/1743-v

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07:11 min

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April 22, 2010

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, , ,
1Department of Immunology,MD Anderson Cancer Center – University of Texas, 2Department of Medicine,University of Miami

Chapters

  • 00:00Title
  • 00:46Introduction
  • 01:12Antigenic Stimulation
  • 02:38Immunofluorescent Staining of Surface Markers for FACS
  • 04:25Intracellular Cytokine Staining
  • 05:27Representative FACS Data
  • 06:29Conclusion

Summary

Automatic Translation

Automatic Translation

We demonstrate the utility of multicolor flow cytometry for detailed phenotypic and functional characterization of total as well as memory subsets of CD4+ and CD8+ T cells in rhesus macaques, the ideal model for HIV/AIDS vaccine studies.

Tags

Multicolor Flow CytometryCellular Immune ResponseRhesus MacaquesHIV-AIDS ModelPathogenesisVaccinesTherapeuticsPhenotypic AnalysisFunctional AnalysisIntracellular Cytokine ProductionCD4+ T CellsCD8+ T CellsMemory SubsetsInterferon Gamma (INF-)Interleukin (IL)-2PMA Plus Ionomycin (PMA+I)Cytokine ProfilesMemory Cells SubsetsAntigen-specific CD4+ T CellsAntigen-specific CD8+ T CellsPBMC SamplesHIV Envelope Peptide Cocktail Vaccine

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