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Biology
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Direct Synthesis of Gold Nanoparticles for Protein Localization Analysis
/
蓝宝石盘载体组件的高压冷冻 （HPF）、冷冻替代固定 （FSF） 和再水化
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JoVE Journal
Biology
High-Pressure Freezing (HPF), Freeze-Substitution Fixation (FSF) and Rehydration of Sapphire Disc-Carrier Assembly
Please note that all translations are automatically generated.
Click here for the English version.
蓝宝石盘载体组件的高压冷冻 （HPF）、冷冻替代固定 （FSF） 和再水化
Direct Synthesis of Gold Nanoparticles for Protein Localization Analysis
DOI:
10.3791/200046-v
•
03:20 min
•
April 28, 2023
•
Zhaodi Jiang
2
1
National Institute of Biological Sciences, Beijing
,
2
Tsinghua Institute of Multidisciplinary Biomedical Research
,
Tsinghua University
Tags
Keywords: High-pressure Freezing
HPF
Freeze-substitution Fixation
FSF
Sapphire Disc-carrier Assembly
1-hexadecene
Freeze-substitution Solution
Acetone
Rehydration
Centrifuge Tube
Tweezers
Liquid Nitrogen
Temperature Control
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