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JoVE Journal
Biology

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OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
 

OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides

Article DOI: 10.3791/2046
June 20th, 2010

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Summary June 20th, 2010

A rapid way is described to gain insights into the structure of polysaccharides in an extracellular matrix. The method takes advantage of the specificity of glycosylhydrolases and the sensitivity of mass spectrometry allowing minute amounts of materials to be analyzed. This technique is adaptable to be used directly on tissue itself.

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