Isolation and Culture of Avian Embryonic Valvular Progenitor Cells

Journal

Biology

JoVE Journal
Biology

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JoVE Journal Biology

Isolation and Culture of Avian Embryonic Valvular Progenitor Cells

DOI:

10.3791/2159-v

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14:02 min

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October 27, 2010

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Gretchen Mahler, Russell Gould, Johnathan Butcher

¹Department of Biomedical Engineering,Cornell University

Chapters

00:00Title
01:07Introduction
01:52Preparing 3D Collagen Gels
03:26Pre-EMT Endocardial Cell Isolation and Culture
07:24Post-EMT AV Cushion Mesenchymal Cell Isolation and Culture
12:06Representative Results
13:29Conclusion

Summary

Automatic Translation

This article will provide a method for isolating and culturing quail or chicken HH14^- valve endocardial cells and HH25 valve cushion mesenchymal cells.

Isolation and Culture of Avian Embryonic Valvular Progenitor Cells

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