Journal
/
Immunology and Infection
/
Specific Marking of HIV-1 Positive Cells using a Rev-dependent Lentiviral Vector Expressing the Green Fluorescent Protein
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Specific Marking of HIV-1 Positive Cells using a Rev-dependent Lentiviral Vector Expressing the Green Fluorescent Protein

Specific Marking of HIV-1 Positive Cells using a Rev-dependent Lentiviral Vector Expressing the Green Fluorescent Protein

DOI:
10.3791/2198-v

•

09:53 min

•

September 23, 2010

•

, ,
1National Center for Biodefense and Infectious Diseases, Department of Molecular and Microbiology,George Mason University

Chapters

  • 00:00Title
  • 01:15Plasmid Transfection to Produce Rev-dependent Lentiviral Particles
  • 04:24Concentrating Viral Particles and Determining Viral Titer
  • 06:15Marking HIV-1 Positive Cells
  • 08:25Identifying HIV-positive Cells
  • 09:30Conclusion

Summary

Automatic Translation

Automatic Translation

We have developed a lentiviral vector that possesses, in addition to the Tat-responsive LTR, the Rev-response element (RRE) that can regulate reporter gene expression in an HIV-1 Tat- and Rev-dependent fashion. The vector permits the specific detection of replicating HIV in living cells via the expression of GFP.

Tags

HIV-1Marking HIV-positive CellsRev-dependent Lentiviral VectorGreen Fluorescent Protein (GFP)HIV-responsive Expression VectorsLong Terminal Repeat (LTR)Tat-responsive LTRCellular Activation StatesLocal Chromatin ActivityHIV-independent ActivityLTR-based ReporterHIV DNA SequencesRev-response ElementHIV Splicing SitesLentiviral Reporter VirusReplicating HIVLiving Cell PopulationsGreen Fluorescence Protein (GFP) ExpressionIn Situ PCRHIV Antigen StainingTherapeutic Genes

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2198?article_type=v

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
check_url/2198?article_type=v

Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach
check_url/2198?article_type=v

In vitro Uncoating of HIV-1 Cores
check_url/2198?article_type=v

New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals
check_url/2198?article_type=v

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
check_url/2198?article_type=v

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay
check_url/2198?article_type=v

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals
check_url/2198?article_type=v

SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
check_url/2198?article_type=v

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells
check_url/2198?article_type=v

Induction of Paralysis and Visual System Injury in Mice by T Cells Specific for Neuromyelitis Optica Autoantigen Aquaporin-4

Read Article